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217620 Anti-CD64 Mouse mAb (10.1)

217620
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
217620-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~72 kDa CD64 protein (FcR1 receptor).
Catalogue Number217620
Brand Family Calbiochem®
References
ReferencesDougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Negative controlHPB-MLT
Positive control4937 cells
Preservative0.5 mg/ml gentamycin sulfate
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Neutralization Studies
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
Application CommentsThe antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman rheumatoid synovial fluid cells/monocytes
ImmunogenHuman
Clone10.1
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CD64 Mouse mAb (10.1) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD64 Mouse mAb (10.1) Certificates of Analysis

TitleLot Number
217620

References

Reference overview
Dougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
ApplicationFlow Cytometry (10 µg/ml)
Frozen Sections (see comments)
Immunoblotting (see comments)
Immunoprecipitation (see comments)
Neutralization Studies (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~72 kDa CD64 (FcR1) protein.
HostMouse
Immunogen speciesHuman
Immunogenhuman rheumatoid synovial fluid cells/monocytes
Clone10.1
IsotypeIgG₁
Specieshuman
Positive control4937 cells
Negative controlHPB-MLT
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Preservative0.5 mg/ml gentamycin sulfate
CommentsThe antibody is reported to block immunoglobulin binding to FcR1. FACS was performed on 5x105 cultured U-937 human tumor cells and analyzed by flow cytometry. Optimal binding concentration tested was 10 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesDougherty, G.J., et al. 1987. Eur. J. Immunol. 17, 1453.