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208910 Anti-Calreticulin (405-417) Rabbit pAb

208910
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ht, H, Mk, M, Porcine, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
208910-50UL Plastic ampoule 50 ul
Description
OverviewRecognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
Catalogue Number208910
Brand Family Calbiochem®
Application Data
Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesJanicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Application NotesImmunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunohistochemistry (see comments)
Application CommentsDoes not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Porcine
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Calreticulin (405-417) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Calreticulin (405-417) Rabbit pAb Certificates of Analysis

TitleLot Number
208910

References

Reference overview
Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-April-2009 JSW
ApplicationImmunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunohistochemistry (see comments)
Application Data
Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
BackgroundCalreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. Calreticulin, which contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
IsotypeIgG
Speciesbovine, canine, hamster, human, monkey, mouse, porcine, rat
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
CommentsDoes not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesJanicki, S., et al. 1997. J. Cell Biol. 139, 485.
Wada, I., et al. 1997. EMBO J. 16, 5420.
Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.