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324402 Anti-Dopamine D₃ Receptor Rabbit pAb

324402
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
324402-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the dopamine D3 receptor in rat brain. Does not cross-react with other dopamine receptors.
Catalogue Number324402
Brand Family Calbiochem®
References
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
Key Applications Immunohistochemistry
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Flow Cytometry (1:100, see application references)
Immunoblotting (1:500)
Immunohistochemistry (1:5000)
Application CommentsThis antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Dopamine D₃ Receptor Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Dopamine D₃ Receptor Rabbit pAb Certificates of Analysis

TitleLot Number
324402

References

Reference overview
Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-July-2007 RFH
ApplicationELISA (see comments)
Flow Cytometry (1:100, see application references)
Immunoblotting (1:500)
Immunohistochemistry (1:5000)
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~50 kDa D3 dopamine receptor protein.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (ASLSQLSSHC) corresponding to amino acids (2-10) of human dopamine D₃ receptor
IsotypeIgG
Specieshuman, rat
Positive controlRat brain
FormLyophilized
FormulationUndiluted serum.
PreservativeNone
CommentsThis antiserum is highly specific for the immunizing peptide sequence. For immunohistochemistry, a 1:5000 dilution of the reconstituted serum demonstrated staining of specific cells in various regions of rat brain sections. Such staining includes dendritic labeling in the molecular layers (cortex layer 1 and molecular layer of the hippocampus) as well as the plasma membranes of neurons in layer 5 of cortex and the glomeruli of the olfactory bulb. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Toxicity Standard Handling
ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
Application referencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.