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05-719 Anti-Phosphatidylserine Antibody, clone 1H6

05-719
200 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtFC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-719
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphatidylserine Antibody, clone 1H6
OverviewSignificance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Background InformationPhosphatidylserine (PS) is a phospholipid (1,2-diacyl-sn-glycerol-(3)-L-phosphoserine) found in the membrane of all plants and animals. PS is most concentrated in the brain, as PS makes up about 10% of the lipid content of neurons. Neural PS is thought to play a role in maintaining nerve cell integrity, enhancing neurotransmitter signal efficiency, and improving nerve cell signal transmission. One of the key features of apoptosis is the expresion of PS on the outer cell membrane. PS is usually found on the inner side of the plasma membrane, but during early stage apoptosis, the PS is translocated to the outer membrane. FITC-labeled annexin V can bind to the exposed PS, providing the basis of most annexin V-based apoptosis assays.
References
Product Information
FormatPurified
Control
  • Brain tissue
PresentationPurified IgG supplied in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 before the addition of glycerol to 30%. Liquid at -20°C.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphatidylserine Antibody, clone 1H6 is an antibody against Phosphatidylserine for use in FC, IH.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
Application NotesFlow Cytometry: 0.2-2 μg/mL of a previous lot detected cell surface phosphatidylserine on Jurkat cells treated with Fas Ligand vesicles (Catalog # 01-210). See page two.

Immunohistochemistry: This antibody has been reported by an independent laboratory to detect phosphatidylserine in rat carotid artery paraffin sections fixed with ethanol/acetone (Mandinov, L., 2003).
Biological Information
ImmunogenLiposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol
Cloneclone 1H6
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes phosphatidylserine (PS) in cell membranes
IsotypeIgG
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
Molecular Weightnone given
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by flow cytometry on Jurkat cells treated with Fas Ligand vesicles (Catalog #01-210)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Phosphatidylserine Antibody, clone 1H6 SDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphatidylserine Antibody, clone 1H6 Certificates of Analysis

TitleLot Number
Anti-Phosphatidylserine, clone 1H6 - 24533 24533
Anti-Phosphatidylserine, clone 1H6 - 2491217 2491217
Anti-Phosphatidylserine, clone 1H6 - 27684 27684
Anti-Phosphatidylserine, clone 1H6 - 28897 28897
Anti-Phosphatidylserine, clone 1H6 - 3169545 3169545
Anti-Phosphatidylserine, clone 1H6 - 3212741 3212741
Anti-Phosphatidylserine, clone 1H6 - 3307801 3307801
Anti-Phosphatidylserine, clone 1H6 - 3438599 3438599
Anti-Phosphatidylserine, clone 1H6 - 3485495 3485495
Anti-Phosphatidylserine, clone 1H6 - 3501406 3501406

References

Reference overviewApplicationPub Med ID
Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration.
Zhao, L; Zabel, MK; Wang, X; Ma, W; Shah, P; Fariss, RN; Qian, H; Parkhurst, CN; Gan, WB; Wong, WT
EMBO molecular medicine  7  1179-97  2015

Show Abstract
26139610 26139610
Lack of association of C-Met-N375S sequence variant with lung cancer susceptibility and prognosis.
Shieh, JM; Tang, YA; Yang, TH; Chen, CY; Hsu, HS; Tan, YH; Salgia, R; Wang, YC
International journal of medical sciences  10  988-94  2013

Show Abstract
23801885 23801885
Metabolism and functions of phosphatidylserine.
Vance, Jean E and Steenbergen, Rineke
Prog. Lipid Res., 44: 207-34 (2005)  2005

Show Abstract
15979148 15979148
The role of phosphatidylserine in recognition and removal of erythrocytes.
Kuypers, F A and de Jong, K
Cell. Mol. Biol. (Noisy-le-grand), 50: 147-58 (2004)  2004

Show Abstract
15095785 15095785
Phosphatidylserine receptor and apoptosis: consequences of a non-ingested meal.
Botto, Marina
Arthritis Res. Ther., 6: 147-50 (2004)  2004

15225357 15225357
Hide and seek: the secret identity of the phosphatidylserine receptor.
Williamson, Patrick and Schlegel, Robert A
J. Biol., 3: 14 (2004)  2004

Show Abstract
15453906 15453906
Early membrane events in polymorphonuclear cell (PMN) apoptosis: membrane blebbing and vesicle release, CD43 and CD16 down-regulation and phosphatidylserine externalization.
Nusbaum, P, et al.
Biochem. Soc. Trans., 32: 477-9 (2004)  2004

Show Abstract
15157165 15157165
Apoptosis: giving phosphatidylserine recognition an assist--with a twist.
Fadok, Valerie A and Henson, Peter M
Curr. Biol., 13: R655-7 (2003)  2003

12932346 12932346
Exposure of platelet membrane phosphatidylserine regulates blood coagulation.
Lentz, Barry R
Prog. Lipid Res., 42: 423-38 (2003)  2003

Show Abstract
12814644 12814644
Copper chelation represses the vascular response to injury
Mandinov, L., et al
Proc Natl Acad Sci U S A, 100:6700-5 (2003)  2003

Immunohistochemistry (Tissue)12754378 12754378

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Alternative Format

Catalogue Number Description
16-256 Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor® 488

Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies