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196810 Bax-Inhibiting Peptide, V5 - Calbiochem

196810
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₇H₅₀N₆O₆S

Products

Catalogue NumberPackaging Qty/Pack
196810-5MG Plastic ampoule 5 mg
Description
OverviewA cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain that offers cytoprotection. Functions as effectively as the Caspase Inhibitor VI (Z-VAD-FMK; Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also effectively blocks caspase-independent necrotic cell death. Shown to be Ku70 competitive, interact with Bax, prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days). Negative control peptide is also available (Cat. No. 196811).
Catalogue Number196810
Brand Family Calbiochem®
SynonymsBIP-V5, H-VPMLK-OH
References
ReferencesYoshida, T., et al. 2004. Biochem. Biophys. Res. Commun. 321, 961.
Sawada, M., et al. 2003. Nature Cell Biol. 5, 352.
Product Information
ATP CompetitiveN
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₂₇H₅₀N₆O₆S
Chemical formulaC₂₇H₅₀N₆O₆S
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
ApplicationBax-Inhibiting Peptide, V5, CAS 579492-81-2, is a cell-permeable pentapeptide based on the Ku70-Bax inhibiting domain. Acts as an effective Inhibitor for Bax-mediated apoptosis (~50-200 µM).
Biological Information
Primary TargetBax mediated apoptosis
Primary Target IC<sub>50</sub>50-200 µM against Bax mediated apoptosis
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceH-Val-Pro-Met-Leu-Lys-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Bax-Inhibiting Peptide, V5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Bax-Inhibiting Peptide, V5 - Calbiochem Certificates of Analysis

TitleLot Number
196810

References

Reference overview
Yoshida, T., et al. 2004. Biochem. Biophys. Res. Commun. 321, 961.
Sawada, M., et al. 2003. Nature Cell Biol. 5, 352.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-April-2008 RFH
SynonymsBIP-V5, H-VPMLK-OH
DescriptionA cell-permeable pentapeptide derived from the Ku70-Bax inhibiting domain that has been shown to offer protection against apoptosis in cells of human, mouse, rant, and porcine origin. Reported to function as effectively as the caspase inhibitor, Z-VAD-FMK (Caspase Inhibitor VI, Cat. No. 219007) for Bax-mediated apoptosis (~50-200 µM). Also reported to effectively block caspase-independent necrotic cell death. Shown to interact with Bax in a Ku70-competitive manner and prevent its conformational change and mitochondrial translocation. Displays extended stability in culture medium (~3 days).
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₇H₅₀N₆O₆S
Peptide SequenceH-Val-Pro-Met-Leu-Lys-OH
Purity≥95% by HPLC
SolubilityH₂O (1 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Storage in glass vials is not recommended as loss of activity may occur.
Toxicity Standard Handling
ReferencesYoshida, T., et al. 2004. Biochem. Biophys. Res. Commun. 321, 961.
Sawada, M., et al. 2003. Nature Cell Biol. 5, 352.