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197320 Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem

197320
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₀₆H₁₉₁N₄₉O₂₃

Products

Catalogue NumberPackaging Qty/Pack
197320-2MG Plastic ampoule 2 mg
Description
OverviewAn all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
Catalogue Number197320
Brand Family Calbiochem®
Synonyms(D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9
References
ReferencesKolluri, S.K., et al. 2008. Cancer Cell 14, 285.
Product Information
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₀₆H₁₉₁N₄₉O₂₃
Chemical formulaC₁₀₆H₁₉₁N₄₉O₂₃
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide Sequence(D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH₂)₅-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem Certificates of Analysis

TitleLot Number
197320

References

Reference overview
Kolluri, S.K., et al. 2008. Cancer Cell 14, 285.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-September-2009 RFH
Synonyms(D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9
DescriptionAn all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
FormLyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₀₆H₁₉₁N₄₉O₂₃
Peptide Sequence(D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH₂)₅-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH₂
Purity≥95% by HPLC
SolubilityH₂O (1 mg/ml) or DMSO (5 mg/ml)
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKolluri, S.K., et al. 2008. Cancer Cell 14, 285.