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197217 Bcl-xL BH4₄₋₂₃, Human, Cell-Permeable - Calbiochem

197217
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
197217-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-xL (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.
Catalogue Number197217
Brand Family Calbiochem®
SynonymsHIV-TAT₄₈₋₅₇-β-Ala-Bcl-xL BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
References
ReferencesChen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem. 276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 3100.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₁₆₅H₂₇₉N₅₉O₄₆
Chemical formulaC₁₆₅H₂₇₉N₅₉O₄₆
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetVoltage-dependent anion channel (VDAC)
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceAc-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Bcl-xL BH4₄₋₂₃, Human, Cell-Permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Bcl-xL BH4₄₋₂₃, Human, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
197217

References

Reference overview
Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem. 276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 3100.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-July-2007
SynonymsHIV-TAT₄₈₋₅₇-β-Ala-Bcl-xL BH4₄₋₂₃, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH
DescriptionA highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-xL (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₆₅H₂₇₉N₅₉O₄₆
Peptide SequenceAc-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml) or 20% Acetic Acid (5 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesChen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem. 276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 3100.