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203189 Biotin-X-NHS, Water-Soluble

203189
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
203189-50MG Plastic ampoule 50 mg
Description
OverviewReadily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.
Catalogue Number203189
Brand Family Calbiochem®
SynonymsSulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
References
ReferencesGretch, D.R., et al. 1987. Anal. Biochem. 163, 270.
Product Information
FormOff white to tan solid
Hill FormulaC₂₀H₂₉N₄O₉S₂ · Na
Chemical formulaC₂₀H₂₉N₄O₉S₂ · Na
Quality LevelMQ100
Applications
Biological Information
Purity≥94% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Biotin-X-NHS, Water-Soluble SDS

Title

Safety Data Sheet (SDS) 

Biotin-X-NHS, Water-Soluble Certificates of Analysis

TitleLot Number
203189

References

Reference overview
Gretch, D.R., et al. 1987. Anal. Biochem. 163, 270.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-May-2008 RFH
SynonymsSulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate
DescriptionWater solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
FormOff white to tan solid
Recommended reaction conditions
Buffers containing amines (such as Tris or glycine) should be avoided because they can compete with the reaction. It is also very important that the stock solution be prepared just prior to addition of the reaction mixture, because these compounds are prone to hydrolysis by water. Water-soluble crosslinkers can be dissolved in water or buffer at a concentration of 10-25 mM. Phosphate buffers may precipitate sulfo-N-hydroxysuccinimide (sulfo-NHS) containing compounds. In addition, the solid crosslinker can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions, but can be minimized by using more concentrated protein solution (greater than 5 mg/ml). If you prefer to work with stock solutions, we recommend that you dissolve the crosslinker in anhydrous DMSO at a concentration of 10-25 mM. This solution can then be diluted into the buffer of your choice just prior to use. General Handling 1. Store desiccated at 4°C. 2. Warm contents to room temperature completely before opening. Allow at least 30 min. 3. Wipe the vial to remove any moisture. 4. After opening, store under nitrogen. 5. Protect from moisture.
Chemical formulaC₂₀H₂₉N₄O₉S₂ · Na
Purity≥94% by HPLC
SolubilitydH₂O (5.5 mg/ml) or DMSO (13 mg/ml)
Storage +2°C to +8°C
Protect from moisture
Do Not Freeze Ok to freeze
Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.
Toxicity Standard Handling
ReferencesGretch, D.R., et al. 1987. Anal. Biochem. 163, 270.