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208783 Calmodulin, Porcine Brain

208783
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
208783-1MG Plastic ampoule 1 mg
Description
OverviewCalmodulin (CaM) purified from porcine brain. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates processes like inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for applications requiring active pure calmodulin.
Catalogue Number208783
Brand Family Calbiochem®
References
ReferencesMassom, L., et al. 1990. Biochemistry 29, 671.
Product Information
Unit of DefinitionOne unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca<sup>2+</sup> in a 3 ml reaction volume at 30°C, pH 7.5.
FormLyophilized
FormulationLyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
Quality LevelMQ100
Applications
Biological Information
Purity>98% by SDS-PAGE
Specific Activity≥ 35,000 U/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Calmodulin, Porcine Brain SDS

Title

Safety Data Sheet (SDS) 

Calmodulin, Porcine Brain Certificates of Analysis

TitleLot Number
208783

References

Reference overview
Massom, L., et al. 1990. Biochemistry 29, 671.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-September-2007 JSW
DescriptionCalmodulin (CaM) purified from porcine brain. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates processes like inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for applications requiring active pure calmodulin.
FormLyophilized
FormulationLyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
Purity>98% by SDS-PAGE
Specific activity≥ 35,000 U/mg protein
Unit definitionOne unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca2+ in a 3 ml reaction volume at 30°C, pH 7.5.
SolubilityH₂O (1 mg/ml)
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMassom, L., et al. 1990. Biochemistry 29, 671.