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208712 Calpain-1, Porcine Erythrocytes

208712
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
208712-1MG Plastic ampoule 1 mg
Description
OverviewNative calpain-1 from porcine erythrocytes. Ca2+-dependent heterodimeric cysteine proteinase with low Ca2+ requirement (EC50= 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin.
Catalogue Number208712
Brand Family Calbiochem®
Synonymsμ-Calpain
References
ReferencesVanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Kampfl, A., et al. 1997. J. Neurotrauma 14, 121.
Johnson, G.V.W., and Gutmann, R.P. 1997. BioEssays 19, 1011.
Bartus, R.T., et al. 1995. Neurol. Res. 17, 249.
Wang, K.K.W., and Yuen, P.-W. 1994. Trends Pharmacol. Sci. 15, 412.
Saito, K., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2628.
Goll, D.E., et al. 1992. BioEssays 14, 549.
Ishii, H., et al. 1992. Biochim. Biophys. Acta 1175, 37.
Melloni, E., and Pontremoli, S. 1989. Trends Neurosci. 12, 438.
Ross, E., and Schatz, G. 1973. Anal. Biochem. 54, 304.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 9 fluorogenic units
EC number3.4.22.17
FormLiquid
FormulationIn 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
PI5.3
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥1000 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42/43

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Short-term storage of aliquots at 4°C or on ice is not recommended.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Calpain-1, Porcine Erythrocytes SDS

Title

Safety Data Sheet (SDS) 

Calpain-1, Porcine Erythrocytes Certificates of Analysis

TitleLot Number
208712

References

Reference overview
Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Kampfl, A., et al. 1997. J. Neurotrauma 14, 121.
Johnson, G.V.W., and Gutmann, R.P. 1997. BioEssays 19, 1011.
Bartus, R.T., et al. 1995. Neurol. Res. 17, 249.
Wang, K.K.W., and Yuen, P.-W. 1994. Trends Pharmacol. Sci. 15, 412.
Saito, K., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2628.
Goll, D.E., et al. 1992. BioEssays 14, 549.
Ishii, H., et al. 1992. Biochim. Biophys. Acta 1175, 37.
Melloni, E., and Pontremoli, S. 1989. Trends Neurosci. 12, 438.
Ross, E., and Schatz, G. 1973. Anal. Biochem. 54, 304.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-May-2010 JSW
Synonymsμ-Calpain
DescriptionNative calpain-1 from porcine erythrocytes. Calpains are a family of calcium-dependent thiol-proteases that degrade a wide variety of cytoskeletal, membrane-associated, and regulatory proteins. The two major isoforms, calpain I (µ-form) and calpain II (m-form), differ in their calcium requirement for activation. Calpain I requires only micromolar amounts of calcium (EC50 = 2 µM), while calpain II requires millimolar amounts (EC50 = 1 mM).

Calpains are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme. The 30 kDa unit is a regulatory subunit and common to both calpain I and calpain II. The 80 kDa unit consists of four domains (I-IV). The 30 kDa unit has two domains (V and VI).

• Domain I is partially removed during autolysis.
• Domain II is the protease domain.
• Domain III exhibits a homology with typical calmodulin binding proteins and interacts with calcium binding domains (IV and VI) and frees domain II for protease activity.
• Domain IV is a calcium binding domain.
• Domain V contains a hydrophobic region and is essential for calpain interaction with membranes.
• Domain VI is a calcium binding domain.

More recently, attention has been focused on the pathological significance of calcium accumulation in the central nervous system following cerebral ischemia and traumatic brain injury. Over-activation of NMDA, kainate, and AMPA receptors in the brain leads to sustained influx in Ca2+ through voltage gated Ca2+ channels. Disturbances in calcium homeostasis result in the activation of several calcium-dependent enzymes including calpains. Over-expression of calpains has been positively linked to both acute and chronic neurodegenerative processes including ischemia, trauma, and Alzheimer's disease. In Alzheimer's disease the ratio of active (76 kDa) to inactive (80 kDa) calpain I is reported to be much higher. Calpain proteolysis is usually the late-stage common pathway towards cell death induced by excitotoxic compounds.
FormLiquid
FormulationIn 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
Concentration Label Please refer to vial label for lot-specific concentration
EC number3.4.22.17
Specific activity≥1000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 9 fluorogenic units
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Short-term storage of aliquots at 4°C or on ice is not recommended.
Toxicity Harmful
ReferencesVanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Kampfl, A., et al. 1997. J. Neurotrauma 14, 121.
Johnson, G.V.W., and Gutmann, R.P. 1997. BioEssays 19, 1011.
Bartus, R.T., et al. 1995. Neurol. Res. 17, 249.
Wang, K.K.W., and Yuen, P.-W. 1994. Trends Pharmacol. Sci. 15, 412.
Saito, K., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 2628.
Goll, D.E., et al. 1992. BioEssays 14, 549.
Ishii, H., et al. 1992. Biochim. Biophys. Acta 1175, 37.
Melloni, E., and Pontremoli, S. 1989. Trends Neurosci. 12, 438.
Ross, E., and Schatz, G. 1973. Anal. Biochem. 54, 304.