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218799 Caspase-6, Human, Recombinant, E. coli

218799
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
218799-100U Plastic ampoule 100 u
Description
OverviewRecombinant, human caspase-6 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
M.W. 11,000 and 18,000 (heterodimer).
Catalogue Number218799
Brand Family Calbiochem®
SynonymsMch2
References
ReferencesLeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218762">Cat. No. 218762</a>) per h at 37°C, pH 7.2.
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥6000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-6, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Caspase-6, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218799

References

Reference overview
LeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsMch2
DescriptionRecombinant, human caspase-6 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 11000 and 18000.
FormLyophilized
Recommended reaction conditions50 mM HEPES, 50 mM NaCl, 10 mM DTT, 10 mM EDTA. 5% glycerol, and 0.1% CHAPS, pH 7.2.
Purity≥95% by SDS-PAGE
Specific activity≥6000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol Ac-VEID-pNA (Cat. No. 218762) per h at 37°C, pH 7.2.
SolubilityReconstitute to 1 unit/µl in PBS, 15% glycerol.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Toxicity Standard Handling
ReferencesLeBlanc, A., et al. 1999. J. Biol. Chem. 274, 23426.
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem. 272, 25719.