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228250 Cholesterol Oxidase, Streptomyces sp.

228250
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
228250-500U Plastic ampoule 500 u
Description
OverviewNative cholesterol oxidase from Streptomyces species. Catalyzes oxidation of cholesterol to cholesterone and hydrogen peroxide (isoelectric points: 6.1 and 7.3). Suitable for determination of total cholesterol when coupled with cholinesterase and peroxidase. Has an optimal pH of 6.0-8.0.
Catalogue Number228250
Brand Family Calbiochem®
SynonymsCholesterol:oxygen oxidoreductase
References
ReferencesGadda, G., et al. 1997. Eur. J. Biochem. 250, 369.
Product Information
CAS number9028-76-6
Activity≥3 units/mg solid
Unit of DefinitionOne unit is defined as the amount of enzyme that will oxidize 1.0 µmol of cholesterol per min at 25°C, pH 7.0.
EC number1.1.3.6
FormYellow lyophilized solid
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥40 units/mg
Physicochemical Information
ContaminantsCatalase: ≤1%; glucose oxidase and uricase: ≤0.01%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Cholesterol Oxidase, Streptomyces sp. SDS

Title

Safety Data Sheet (SDS) 

Cholesterol Oxidase, Streptomyces sp. Certificates of Analysis

TitleLot Number
228250

References

Reference overview
Gadda, G., et al. 1997. Eur. J. Biochem. 250, 369.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2008 JSW
SynonymsCholesterol:oxygen oxidoreductase
DescriptionNative cholesterol oxidase from streptomyces species. Catalyzes oxidation of cholesterol to cholesterone and hydrogen peroxide (isoelectric points: 6.1 and 7.3). Suitable for determination of total cholesterol when coupled with cholinesterase and peroxidase. Has an optimal pH of 6.0-8.0.
FormYellow lyophilized solid
CAS number9028-76-6
EC number1.1.3.6
ContaminantsCatalase: ≤1%; glucose oxidase and uricase: ≤0.01%.
Specific activity≥40 units/mg
Activity≥3 units/mg solid
Unit definitionOne unit is defined as the amount of enzyme that will oxidize 1.0 µmol of cholesterol per min at 25°C, pH 7.0.
SolubilityH₂O (20 mg/ml)
Storage -20°C
Hygroscopic
Protect from moisture
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesGadda, G., et al. 1997. Eur. J. Biochem. 250, 369.