Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

219365 Coagulation Factor V, Human Plasma

219365
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
219365-100UG Plastic ampoule 100 μg
Description
OverviewNative coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca2+, and membrane phospholipids are cofactors that form a "prothrombinase" complex with Factor X, enabling its proteolytic activation of prothrombin to thrombin.
Catalogue Number219365
Brand Family Calbiochem®
SynonymsProaccelerin, Labile Factor, Ac-globulin, Ac-g
References
ReferencesWalker, F.J. 1992. J. Biol. Chem. 267, 19896.
Monkovic, D.D., and Tracy, P.B. 1990. J. Biol. Chem. 265, 17132.
Krishnaswamy, S., et al. 1989. J. Biol. Chem. 264, 3160.
Lee, C.D., and Mann, K.G. 1989. Blood 73, 185.
Krieg, U.C., et al. 1987. Biochemistry 26, 103.
Product Information
CAS number9001-24-5
FormLiquid
FormulationIn 50% glycerol/H₂O (v/v).
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥20 units/mg protein (clotting assay). Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Do not store below -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Coagulation Factor V, Human Plasma SDS

Title

Safety Data Sheet (SDS) 

Coagulation Factor V, Human Plasma Certificates of Analysis

TitleLot Number
219365

References

Reference overview
Walker, F.J. 1992. J. Biol. Chem. 267, 19896.
Monkovic, D.D., and Tracy, P.B. 1990. J. Biol. Chem. 265, 17132.
Krishnaswamy, S., et al. 1989. J. Biol. Chem. 264, 3160.
Lee, C.D., and Mann, K.G. 1989. Blood 73, 185.
Krieg, U.C., et al. 1987. Biochemistry 26, 103.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-April-2008 RFH
SynonymsProaccelerin, Labile Factor, Ac-globulin, Ac-g
DescriptionNative coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca2+, and membrane phospholipids are cofactors which form a "prothrombinase" complex with Factor Xa, enabling its proteolytic activation of prothrombin to thrombin.
FormLiquid
FormulationIn 50% glycerol/H₂O (v/v).
Concentration Label Please refer to vial label for lot-specific concentration
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number9001-24-5
Purity≥95% by SDS-PAGE
Specific activity≥20 units/mg protein (clotting assay). Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Do not store below -20°C.
Toxicity Standard Handling
Merck USA index14, 3919
ReferencesWalker, F.J. 1992. J. Biol. Chem. 267, 19896.
Monkovic, D.D., and Tracy, P.B. 1990. J. Biol. Chem. 265, 17132.
Krishnaswamy, S., et al. 1989. J. Biol. Chem. 264, 3160.
Lee, C.D., and Mann, K.G. 1989. Blood 73, 185.
Krieg, U.C., et al. 1987. Biochemistry 26, 103.