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234401 Complement C1q-Depleted Serum, Human

234401
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
234401-1ML Plastic ampoule 1 ml
Description
OverviewHuman serum that has been depleted of C1q complement component.
Catalogue Number234401
Brand Family Calbiochem®
References
Product Information
Unit of Definition<b>Classical Pathway Activity:</b> One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. <b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. <b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
FormClear, amber solution
FormulationIn PBS, ≤0.1% sodium azide, pH 7.4.
PreservativeNone
Quality LevelMQ100
Applications
Application CommentsNo C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:

CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
• Recommended volume of C1q-Dpl serum per assay
• C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
• Input of purified C1q to yield 1 C1qH50:
• C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
• Input of C1q in NHS Complement standard to yield 1 C1qH50
• Ratio
• Background A412 EA blank reading at the recommended input of C1q-Dpl

CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
• CH50/ml NHS Standard
• CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
• Ratio

ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
• APH50/ml NHS Standard
• Input of NHS Standard to yield 1 APH50
• APH50/ml C1q-Dpl
• Input of C1q-Dpl to yield 1 APH50
• AP Ratio
Biological Information
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsC1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Complement C1q-Depleted Serum, Human SDS

Title

Safety Data Sheet (SDS) 

Complement C1q-Depleted Serum, Human Certificates of Analysis

TitleLot Number
234401

Citations

Title
  • Paramita Baruah, et al. (2006) The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells. Journal of Leukocyte Biology in press,.
  • Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision09-June-2008 RFH
    DescriptionHuman serum that has been depleted of C1q complement component. C1q-depleted human serum, sterile filtered through a 0.22 µm filter. This product has the following specifications:
    FormClear, amber solution
    FormulationIn PBS, ≤0.1% sodium azide, pH 7.4.
    Concentration Label Please refer to vial label for lot-specific concentration
    Recommended reaction conditionsClassical Pathway Activity Recommended volume C1q-Dpl serum per assay: ≤ 40 µl C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl: ≥ 12,500 C1qH50/mg C1q input/C1qH50: ≤80 ng C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl: ≥3,500 units/ml Input of C1q in NHS Complement standard to yield 1 C1qH50: ≤ 20 ng C1q in NHS Standard Ratio: ≥ 0.20 (C1qH50/mg C1q)/(C1qH50/mg C1q in NHS Std) Background A412 EA blank reading at the recommended input of C1q-Dpl: ≤ 0.250 CH50 Functional Activity upon Reconstitution CH50/ml NHS Standard: ≥75 CH50 units/ml CH50/ml C1q-Dpl reconstituted with 100 µg C1q/ml: ≥40 CH50 units/ml Ratio: ≥0.50 (CH50/ml C1q-Dpl + C1q)/(CH50/ml NHS Std) Alternative Pathway Activity APH50 units/ml NHS Complement Standard: ≥50 APH50 units/ml Input of NHS Standard to yield 1 APH50: ≤20 µl APH50 units/ml C1q-Dpl: ≥50 APH50 units/ml Input of C1q-Dpl to yield 1 APH50: ≤20 µl AP Ratio: ≥0.50 (APH50/ml C1q-Dpl)/(APH50/ml NHS Std)
    SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
    ContaminantsC1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
    Unit definitionClassical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
    PreservativeNone
    CommentsNo C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum. The following information will vary from lot to lot and can be found on the certificate of analysis:

    CLASSICAL PATHWAY ACTIVITY: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 minutes at 37°C in a total reaction volume of 500 μl GVB++.
    • Recommended volume of C1q-Dpl serum per assay
    • C1qH50 units/mg purified C1q at the recommended input of C1q-Dpl
    • Input of purified C1q to yield 1 C1qH50:
    • C1qH50 units/ml NHS Complement standard at the recommended input of C1q-Dpl
    • Input of C1q in NHS Complement standard to yield 1 C1qH50
    • Ratio
    • Background A412 EA blank reading at the recommended input of C1q-Dpl

    CH50 FUNCTIONAL ACTIVITY UPON RECONSTITUTION: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 minutes at 37°C in a total reaction volume of 1.5 ml GVB++.
    • CH50/ml NHS Standard
    • CH50/ml C1q-Dpl reconstituted with 100 μg C1q/ml:
    • Ratio

    ALTERNATIVE PATHWAY ACTIVITY: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 minutes at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
    • APH50/ml NHS Standard
    • Input of NHS Standard to yield 1 APH50
    • APH50/ml C1q-Dpl
    • Input of C1q-Dpl to yield 1 APH50
    • AP Ratio
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    Citation
  • Paramita Baruah, et al. (2006) The tissue pentraxin PTX3 limits C1q-mediated complement activation and phagocytosis of apoptotic cells by dendritic cells. Journal of Leukocyte Biology in press,.
  • Jose Yuste, et al. (2006) Roles of the Alternative Complement Pathway and C1q during Innate Immunity to Streptococcus pyogenes. Journal of Immunology 176, 6112-6120.