Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

204885 Complement C3, Human

204885
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
204885-250UG Plastic ampoule 250 μg
Description
OverviewNative, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115 kDa (α) and 75 kDa (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77 resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. This covalent attachment of C3b to target acceptors is required for continuation of complement activation.
Catalogue Number204885
Brand Family Calbiochem®
References
ReferencesLambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol. 23, 1237.
Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.
Product Information
CAS number80295-41-6
FormLiquid
FormulationIn PBS.
Quality LevelMQ100
Applications
Biological Information
Biological activity>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.
Purity≥85% by SDS-PAGE
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Physicochemical Information
ContaminantsIgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin: ≤trace amounts
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Complement C3, Human SDS

Title

Safety Data Sheet (SDS) 

Complement C3, Human Certificates of Analysis

TitleLot Number
204885

References

Reference overview
Lambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol. 23, 1237.
Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.

Citations

Title
  • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision29-June-2018 JSW
    DescriptionNative, human C3 complement component. Glycoprotein composed of two non-identical disulfide-bonded subunits with M.W. of 115,000 (α) and 75,000 (β). Present in normal human serum at 1.25 mg/ml. Classical and alternative activation pathways of complement converge at the C3 step. Activation via either pathway can result in assembly of C3-cleaving enzymes (C3 convertases) on target surfaces. Both C3 convertases cleave the C3 α-chain at peptide bond 77, resulting in production of C3a (M.W. 9083) and C3b fragments (M.W. 180,000). Released C3a peptide is one of the three complement anaphylatoxins. The nascent C3b fragment can form a covalent ester bond with target surface. The covalent attachment of C3b to target acceptors is required for continuation of complement activation.
    FormLiquid
    FormulationIn PBS.
    SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
    CAS number80295-41-6
    Purity≥85% by SDS-PAGE
    ContaminantsIgG, IgA, IgM, C4, functionally active C5, albumin or ceruloplasmin: ≤trace amounts
    Biological activity>3,000 C₃H₅0 units/mg; ≥60% of the C3 activity in normal human serum on a mg/mg basis.
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesLambris, J.D. and Muller-Eberhard, H.J. 1986. Mol. Immunol. 23, 1237.
    Tack, B.F., et al. 1980. Methods Enzymol. 80, 64.
    Citation
  • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.