Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

204886 Complement C4, Human

204886
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
204886-250UG Plastic ampoule 250 μg
Description
OverviewNative, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. of 93 kDa (α), 75 kDa (β), and 32 kDa (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in production of C4a (M.W. 8740) and C4b fragments (M.W. 193 kDa). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
Catalogue Number204886
Brand Family Calbiochem®
References
ReferencesJanatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
Product Information
CAS number80295-48-3
FormLiquid
FormulationIn PBS, pH 7.2.
Quality LevelMQ100
Applications
Biological Information
Biological activity≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
Purity≥90% by SDS-PAGE
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity>3X10⁵ C4H50 units/mg
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Complement C4, Human SDS

Title

Safety Data Sheet (SDS) 

Complement C4, Human Certificates of Analysis

TitleLot Number
204886

References

Reference overview
Janatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.

Citations

Title
  • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-May-2008 RFH
    DescriptionNative, human C4 complement component. Glycoprotein composed of three non-identical subunits of M.W. 93,000 (α), 75,000 (β), and 32,000 (γ) linked by disulfide bonds. Present in normal human serum at 400 µg/ml. On activation of complement via the classical pathway, the C1s subcomponent of the C1 complex is converted to an active serine protease that cleaves the C4 α-chain at peptide bond 77, resulting in the production of C4a (M.W. 8740) and C4b fragments (M.W. 193,000). The released C4a peptide is one of the three complement-derived anaphylatoxins. The nascent C4b fragment can form a covalent ester bond with target surfaces. This covalent attachment of C4b to target acceptors is required for continuation of activation via classical pathway.
    FormLiquid
    FormulationIn PBS, pH 7.2.
    Concentration Label Please refer to vial label for lot-specific concentration
    SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
    CAS number80295-48-3
    Purity≥90% by SDS-PAGE
    Specific activity>3X10⁵ C4H50 units/mg
    Biological activity≥60% of the C4 activity present in freshly drawn normal human serum on a mg to mg basis
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesJanatova, J. 1983. Ann. N.Y. Acad. Sci. 421, 218.
    Hammer, C.H., et al. 1981. J. Biol. Chem. 256, 3995.
    Citation
  • Inal, J.M. and Schifferli J.A., 2002. Journal of Immunology 168, 5213.