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234610 Congo Red, High Purity - CAS 573-58-0 - Calbiochem

234610
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₃₂H₂₂N₆O₆S₂ · 2Na573-58-0

Products

Catalogue NumberPackaging Qty/Pack
234610-500MG Glass bottle 500 mg
Description
OverviewAn amyloidophylic dye that specifically stains stacked β sheet aggregates. Useful for staining and visual detection of amyloid deposits. Does not bind to non-polymerized amyloid peptide. Inhibits the accumulation of the scrapie-associated, protease-resistant isoform of protein PrP without affecting the metabolism of the normal isoform. It is also shown to inhibit Pot1 Binding to Telomeric DNA.
Catalogue Number234610
Brand Family Calbiochem®
Synonyms3,3ʹ-[(1,1ʹ-Biphenyl)-4,4ʹ-diylbis(azo)]bis(4-amino-1-naphthalenesulfonic Acid) 2Na
References
ReferencesAltschuler, S.E., et al. 2012. Biochemistr in press.
Khurana, R., et al. 2001. J. Biol. Chem. 276, 22715.
Fezoui, Y., et al. 2000. Amyloid 7, 166.
Fezoui, Y., et al. 2000. Nat. Struct. Biol. 7, 1095.
Kramport, M., et al. 2000. Chem. Biol. 7, 855.
Kuner, P., et al. 2000. J. Biol. Chem. 275, 1673.
Caspi, S., et al. 1998. J. Biol. Chem. 273, 3484.
Caughey, B., and Race, A.E. 1992. J. Neurochem. 59, 768.
Taylor, D.L., et al. 1974. J. Histochem. Cytochem. 22, 1105.
Product Information
CAS number573-58-0
ATP CompetitiveN
FormRust crystalline solid
Hill FormulaC₃₂H₂₂N₆O₆S₂ · 2Na
Chemical formulaC₃₂H₂₂N₆O₆S₂ · 2Na
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetAn amyloidophylic dye that specifically stains stacked β sheet aggregates
Purity≥75% (dye content)
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSQK1400000
Safety Information
S PhraseS: 25

Avoid contact with eyes.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at room temperature (20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Congo Red, High Purity - CAS 573-58-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Congo Red, High Purity - CAS 573-58-0 - Calbiochem Certificates of Analysis

TitleLot Number
234610

References

Reference overview
Altschuler, S.E., et al. 2012. Biochemistr in press.
Khurana, R., et al. 2001. J. Biol. Chem. 276, 22715.
Fezoui, Y., et al. 2000. Amyloid 7, 166.
Fezoui, Y., et al. 2000. Nat. Struct. Biol. 7, 1095.
Kramport, M., et al. 2000. Chem. Biol. 7, 855.
Kuner, P., et al. 2000. J. Biol. Chem. 275, 1673.
Caspi, S., et al. 1998. J. Biol. Chem. 273, 3484.
Caughey, B., and Race, A.E. 1992. J. Neurochem. 59, 768.
Taylor, D.L., et al. 1974. J. Histochem. Cytochem. 22, 1105.

Brochure

Title
Alzheimer's Disease Brochure & Technical Guide
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-September-2012 JSW
Synonyms3,3ʹ-[(1,1ʹ-Biphenyl)-4,4ʹ-diylbis(azo)]bis(4-amino-1-naphthalenesulfonic Acid) 2Na
DescriptionAn amyloidophylic dye that specifically stains stacked β sheet aggre-gates. Useful for staining and visual detection of amyloid deposits in nerve tissue. Binds to amyloid fibrils but not to non-polymerized amyloid peptide. Inhibits the accumulation of the scrapie-associated, protease-resistant isoform of protein PrP without affecting the normal isoform possibly through overstabilization of the conformation. It is also shown to inhibit Pot1 Binding to Telomeric DNA.
FormRust crystalline solid
Recommended reaction conditionsDissolve 0.5 g Congo Red in 80 ml of absolute ethanol. To this solution add 20 ml of 10% NaCl solution (2 g/20 ml of water). Filter before use. Immerse coverslips containing cells or sections into this solution for 3 min at room temperature. Wash with several exchanges of distilled H2O until the water runs is clear. Dehydrate with ascending alcohol solutions (50%, 70%, 80%, 95% x 2, 100% x 2). Clear with 100% xylene. Mount coverslip onto slide using Permount or other suitable organic mounting medium and view under a microscope. Amyloid deposits will appear red to pink-red. With a polarizing microscope, amyloid deposits show an apple-green birefringence.
CAS number573-58-0
RTECSQK1400000
Chemical formulaC₃₂H₂₂N₆O₆S₂ · 2Na
Structure formulaStructure formula
Purity≥75% (dye content)
SolubilityEthanol or H₂O (10 mg/ml)
Storage +15°C to +30°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at room temperature (20°C).
Toxicity Standard Handling
Merck USA index14, 2498
ReferencesAltschuler, S.E., et al. 2012. Biochemistr in press.
Khurana, R., et al. 2001. J. Biol. Chem. 276, 22715.
Fezoui, Y., et al. 2000. Amyloid 7, 166.
Fezoui, Y., et al. 2000. Nat. Struct. Biol. 7, 1095.
Kramport, M., et al. 2000. Chem. Biol. 7, 855.
Kuner, P., et al. 2000. J. Biol. Chem. 275, 1673.
Caspi, S., et al. 1998. J. Biol. Chem. 273, 3484.
Caughey, B., and Race, A.E. 1992. J. Neurochem. 59, 768.
Taylor, D.L., et al. 1974. J. Histochem. Cytochem. 22, 1105.