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2384 Creatine Phosphokinase, Rabbit Skeletal Muscle

2384
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
2384-20MG Plastic ampoule 20 mg
Description
OverviewNative creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.
Catalogue Number2384
Brand Family Calbiochem®
SynonymsrCPK
References
ReferencesBessman, S.P. 1987. Anal. Biochem. 161, 519.
Product Information
CAS number9001-15-4
Activity≥300 units/mg (at 25°C); 750 units/mg (at 37°C)
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.
EC number2.7.3.2
FormLyophilized
Hygroscopic Hygroscopic
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
ContaminantsATPase, hexokinase, myokinase: <0.001%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Creatine Phosphokinase, Rabbit Skeletal Muscle SDS

Title

Safety Data Sheet (SDS) 

Creatine Phosphokinase, Rabbit Skeletal Muscle Certificates of Analysis

TitleLot Number
2384

References

Reference overview
Bessman, S.P. 1987. Anal. Biochem. 161, 519.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 JSW
SynonymsrCPK
DescriptionNative creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.
FormLyophilized
CAS number9001-15-4
EC number2.7.3.2
ContaminantsATPase, hexokinase, myokinase: <0.001%
Activity≥300 units/mg (at 25°C); 750 units/mg (at 37°C)
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.
Solubility0.5% NaHCO₃, pH 9.0 or 0.1 M Imidazole Buffer, pH 6.6
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.
Toxicity Standard Handling
ReferencesBessman, S.P. 1987. Anal. Biochem. 161, 519.