Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

251500 DAF-2 - CAS 205391-01-1 - Calbiochem

251500
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₂₀H₁₄N₂O₅205391-01-1

Products

Catalogue NumberPackaging Qty/Pack
251500-1MG Plastic ampoule 1 mg
Description
OverviewA highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
Catalogue Number251500
Brand Family Calbiochem®
Synonyms4,5-Diaminofluorescein
References
ReferencesBerkels, R., et al. 2000. Cell Calcium 27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Product Information
CAS number205391-01-1
ATP CompetitiveN
DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
FormColorless to pale yellow solution
FormulationA 5 mM (1 mg in 550 µl) solution in DMSO.
Hill FormulaC₂₀H₁₄N₂O₅
Chemical formulaC₂₀H₁₄N₂O₅
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetFluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
Purity≥98% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

DAF-2 - CAS 205391-01-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

DAF-2 - CAS 205391-01-1 - Calbiochem Certificates of Analysis

TitleLot Number
251500

References

Reference overview
Berkels, R., et al. 2000. Cell Calcium 27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-May-2020 JSW
Synonyms4,5-Diaminofluorescein
DescriptionA highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
FormColorless to pale yellow solution
FormulationA 5 mM (1 mg in 550 µl) solution in DMSO.
CAS number205391-01-1
Chemical formulaC₂₀H₁₄N₂O₅
Structure formulaStructure formula
Purity≥98% by HPLC
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesBerkels, R., et al. 2000. Cell Calcium 27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.