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322133 DSP, Water-Soluble

322133
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥94% by HPLC

Products

Catalogue NumberPackaging Qty/Pack
322133-100MG Plastic ampoule 100 mg
Description
OverviewBifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups.
Catalogue Number322133
Brand Family Calbiochem®
Synonyms3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
References
ReferencesHoward, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
Yamada, H., et al. 1981. Biochem. 20, 4836.
Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.
Product Information
FormOff-white to light tan solid
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥94% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

DSP, Water-Soluble SDS

Title

Safety Data Sheet (SDS) 

DSP, Water-Soluble Certificates of Analysis

TitleLot Number
322133

References

Reference overview
Howard, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
Yamada, H., et al. 1981. Biochem. 20, 4836.
Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-June-2008 RFH
Synonyms3,3-Dithio-bis-(sulfosuccinimidyl)propionate, DTSSP, Sulfo-DSP
DescriptionBifunctional cross-linker. The disulfide bond can be cleaved with 1% NaBH4, 50 mM DTT, or 100 mM β-mercaptoethanol. Has been used to immobilize proteins on supports containing amino groups. Chemical crosslinkers are used to covalently modify proteins for studying ligand receptor interactions, conformational changes in tertiary structure, or for protein labeling. Crosslinkers are divided into homobifunctional crosslinkers, containing two identical reactive groups, or heterobifunctional crosslinkers, containing two different reactive groups. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.
FormOff-white to light tan solid
Structure formulaStructure formula
Purity≥94% by HPLC
SolubilityH₂O (6 mg/ml)
Storage +2°C to +8°C
Protect from moisture
Do Not Freeze Ok to freeze
Special InstructionsFollowing each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid DSP can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
Toxicity Irritant
ReferencesHoward, A.D., et al. 1985. J. Biol. Chem. 260, 10833.
Lee, W.T., and Conrad, D.H. 1985. J. Immunol. 134, 518.
D'Souza, S.E., et al. 1988. J. Biol. Chem. 263, 3943.
Yamada, H., et al. 1981. Biochem. 20, 4836.
Annunziato, M.E., et al. 1993. Bioconjugate Chem. 4, 212.