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322337 Dvl-PDZ Domain Inhibitor, Peptide Pen-N3

322337
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₆₃H₂₅₆N₄₈O₃₇S

Products

Catalogue NumberPackaging Qty/Pack
322337-2MG Glass bottle 2 mg
Description
OverviewA 22-amino acid PDZ domain-binding carboxamide toward Dvl (dishevelled) family proteins is fused at the N-terminal with a 16-mer antennapedia homeodomain-derived cell-permeable acetamide to facilitate its use in cellular studies. Shown to selectively inhibit the upregulation of cellular β-catenin accumulation and transcription activity upon Wnt3a stimulation (IC50 = 11 µM) or Dvl1/2/3 overexpression in HEK293 cultures, but not the Wnt3a- or Dvl1/2/3-independent, constitutively active Wnt pathway signaling in the APC-mutant HCT-15 colon cancer cells. Unlike Box5, the PDZ-interacting sequence in Pen-N3, originally identified via a random phage display screening, is not derived from Wnt or other known cellular proteins.
Catalogue Number322337
Brand Family Calbiochem®
SynonymsWnt Pathway Inhibitor III, Ac-RQIKIWFQNRRMKWKK-GGGEIVLWSDIP-NH₂, Dishevelled PDZ Domain Inhibitor
References
ReferencesZhang, Y., et al. 2009. Nat. Chem. Biol. 5, 217.
Product Information
FormLyophilized white solid
FormulationSupplied as a trifluoroacetate salt
Hill FormulaC₁₆₃H₂₅₆N₄₈O₃₇S
Chemical formulaC₁₆₃H₂₅₆N₄₈O₃₇S
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Peptide SequenceAc-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Gly-Gly-Gly-Glu-Ile-Val-Leu-Trp-Ser-Asp-Ile-Pro-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Dvl-PDZ Domain Inhibitor, Peptide Pen-N3 SDS

Title

Safety Data Sheet (SDS) 

Dvl-PDZ Domain Inhibitor, Peptide Pen-N3 Certificates of Analysis

TitleLot Number
322337

References

Reference overview
Zhang, Y., et al. 2009. Nat. Chem. Biol. 5, 217.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-June-2010 RFH
SynonymsWnt Pathway Inhibitor III, Ac-RQIKIWFQNRRMKWKK-GGGEIVLWSDIP-NH₂, Dishevelled PDZ Domain Inhibitor
DescriptionA 22-amino acid PDZ domain-binding carboxamide toward Dvl (dishevelled) family proteins is fused at the N-terminal with a 16-mer antennapedia homeodomain-derived cell-permeable acetamide to facilitate its use in cellular studies. Shown to selectively inhibit the upregulation of cellular β-catenin accumulation and transcription activity upon Wnt3a stimulation (IC50 = 11 µM) or Dvl1/2/3 overexpression in HEK293 cultures, but not the Wnt3a- or Dvl1/2/3-independent, constitutively active Wnt pathway signaling in the APC-mutant HCT-15 colon cancer cells. Unlike Box5, the PDZ-interacting sequence in Pen-N3, originally identified via a random phage display screening, is not derived from Wnt or other known cellular proteins.
FormLyophilized white solid
FormulationSupplied as a trifluoroacetate salt
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₆₃H₂₅₆N₄₈O₃₇S
Peptide SequenceAc-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Gly-Gly-Gly-Glu-Ile-Val-Leu-Trp-Ser-Asp-Ile-Pro-NH₂
Purity≥97% by HPLC
SolubilityDMSO (5 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Standard Handling
ReferencesZhang, Y., et al. 2009. Nat. Chem. Biol. 5, 217.