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205615 E-Cadherin Upregulator, ResEcad - Calbiochem

205615
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₇H₁₇N₃O₃

Products

Catalogue NumberPackaging Qty/Pack
205615-10MG Glass bottle 10 mg
Description
OverviewA cell-permeable isoxazolocarboxamide compound that enhances the E-cadherin protein level in lung squamous cell carcinoma H520 and colorectal adenocarcinoma SW620 cells (6.3- and 10-fold, respectively; 10 µM for 24 h) in a dose-dependent manner (EC50 = 1.25 and 2.13 µM, respectively), resulting in an effective culture invasiveness reduction (by ~50% in SW620 invasion assays). The observed cellular E-cadherin protein upregulation correlates well with an increase in both cellular E-cadherin gene CHD1 transcription (10.2-fold increase in SW620 by RNA-Seq analysis;10 µM for 24 h) and histone H4 Lys9 acetylation (19- and 57-fold enhancement in H529 and SW620 cells, respectively; 10 µM for 24 h), although evidence indicates no direct inhibition of HDACs 1-11 activity by the compound and other cellular factors involved in histone acetylation regulation are the likely candidates as the direct drug target(s).
Catalogue Number205615
Brand Family Calbiochem®
Synonyms5-(Furan-2-yl)-N-(pyridine-4-yl)butyl)isoxazole-3-carboxamide, E-Cadherin Expression Restorer
References
ReferencesStoops, S.L., et al. 2011. ACS Chem. Biol. 6, 452.
Product Information
FormOff-white powder
Hill FormulaC₁₇H₁₇N₃O₃
Chemical formulaC₁₇H₁₇N₃O₃
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

E-Cadherin Upregulator, ResEcad - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

E-Cadherin Upregulator, ResEcad - Calbiochem Certificates of Analysis

TitleLot Number
205615

References

Reference overview
Stoops, S.L., et al. 2011. ACS Chem. Biol. 6, 452.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-November-2011 RFH
Synonyms5-(Furan-2-yl)-N-(pyridine-4-yl)butyl)isoxazole-3-carboxamide, E-Cadherin Expression Restorer
DescriptionA cell-permeable isoxazolocarboxamide compound that enhances the E-cadherin protein level in lung squamous cell carcinoma H520 and colorectal adenocarcinoma SW620 cells (6.3- and 10-fold, respectively; 10 µM for 24 h) in a dose-dependent manner (EC50 = 1.25 and 2.13 µM, respectively), resulting in an effective culture invasiveness reduction (by ~50% in SW620 invasion assays). The observed cellular E-cadherin protein upregulation correlates well with an increase in both cellular E-cadherin gene CHD1 transcription (10.2-fold increase in SW620 by RNA-Seq analysis;10 µM for 24 h) and histone H4 Lys9 acetylation (19- and 57-fold enhancement in H529 and SW620 cells, respectively; 10 µM for 24 h), although evidence indicates no direct inhibition of HDACs 1-11 activity by the compound and other cellular factors involved in histone acetylation regulation are the likely candidates as the direct drug target(s).
FormOff-white powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₇H₁₇N₃O₃
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (50 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Regulatory Review
ReferencesStoops, S.L., et al. 2011. ACS Chem. Biol. 6, 452.