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328000 ERK Activation Inhibitor Peptide I, Cell-Permeable - Calbiochem

328000
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₈₆H₁₅₃N₁₉O₁₇S

Products

Catalogue NumberPackaging Qty/Pack
328000-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable and reversible stearated 13-amino acid peptide corresponding to the N-terminus of MEK1 (MAPKK) that acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of Elk1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 2.5 µM). Potently blocks ERK activation in PMA-stimulated NIH 3T3 cells and NGF-treated PC12 cells (IC50 = 13 µM). Does not affect the activation of JNKs or p38.
Catalogue Number328000
Brand Family Calbiochem®
SynonymsSte-MEK1₁₃, Ste-MPKKKPTPIQLNP-NH₂, Erk Inhibitor IV
References
ReferencesKelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.
Product Information
ATP CompetitiveN
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₈₆H₁₅₃N₁₉O₁₇S
Chemical formulaC₈₆H₁₅₃N₁₉O₁₇S
Hygroscopic Hygroscopic
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetMEK
Primary Target IC<sub>50</sub>2.5 µM
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Peptide SequenceSte-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

ERK Activation Inhibitor Peptide I, Cell-Permeable - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

ERK Activation Inhibitor Peptide I, Cell-Permeable - Calbiochem Certificates of Analysis

TitleLot Number
328000

References

Reference overview
Kelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-January-2010 RFH
SynonymsSte-MEK1₁₃, Ste-MPKKKPTPIQLNP-NH₂, Erk Inhibitor IV
DescriptionA cell-permeable, reversible, and stearated, 13-amino acid peptide corresponding to the N-terminus of MAPK/ERK kinase (MEK). Acts as a specific inhibitor of ERK activation and blocks the transcriptional activity of ELK1. Selectively binds to ERK2 and prevents its interaction with MEK (IC50 = 2.5 µM). Shown to block ERK activity in PMA-stimulated NIH3T3 cells and NGF-treated PC12 cells (IC50 = 13 µM). Does not affect the activation of either JNKs or p38 MAP kinase.
FormWhite lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₈₆H₁₅₃N₁₉O₁₇S
Peptide SequenceSte-Met-Pro-Lys-Lys-Lys-Pro-Thr-Pro-Ile-Gln-Leu-Asn-Pro-NH₂ (Ste = stearated)
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
Storage -20°C
Hygroscopic
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKelemen, B.R., et al. 2002. J. Biol. Chem. 277, 8741.