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330005 EST - CAS 88321-09-9 - Calbiochem

330005
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₇H₃₀N₂O₅88321-09-9

Products

Catalogue NumberPackaging Qty/Pack
330005-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable, irreversible inhibitor of cysteine proteases. Similar to E-64 (Cat. No. 324890) but devoid of charged groups. Reported to inhibit calpain-1 activation. The inhibitory activity of EST has been attributed to E-64c, the free acid formed by hydrolysis of the ester in vivo. Used in animal models of muscular dystrophy.
Catalogue Number330005
Brand Family Calbiochem®
SynonymsLoxistatin, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d
References
ReferencesInomata, M., et al. 1996. Arch. Biochem. Biophys. 328, 129.
Mehdi, S. 1991. Trends Biochem. Sci. 16, 150.
McGowan, E.B., et al. 1989. Biochem. Biophys. Res. Commun. 158, 432.
Tamai, M., et al. 1987. J. Pharmacobiodyn. 10, 678.
Komatsu, K., et al. 1986. Exp. Neurol. 91, 23.
Tamai, M., et al. 1986. J. Pharmacobiodyn. 9, 672.
Product Information
CAS number88321-09-9
ATP CompetitiveN
FormWhite solid
Hill FormulaC₁₇H₃₀N₂O₅
Chemical formulaC₁₇H₃₀N₂O₅
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetcalpain-1
Primary Target IC<sub>50</sub>10-100 µM for calpain-1
Purity≥97% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSRR0404300
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

EST - CAS 88321-09-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

EST - CAS 88321-09-9 - Calbiochem Certificates of Analysis

TitleLot Number
330005

References

Reference overview
Inomata, M., et al. 1996. Arch. Biochem. Biophys. 328, 129.
Mehdi, S. 1991. Trends Biochem. Sci. 16, 150.
McGowan, E.B., et al. 1989. Biochem. Biophys. Res. Commun. 158, 432.
Tamai, M., et al. 1987. J. Pharmacobiodyn. 10, 678.
Komatsu, K., et al. 1986. Exp. Neurol. 91, 23.
Tamai, M., et al. 1986. J. Pharmacobiodyn. 9, 672.

Citations

Title
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision10-July-2013 JSW
    SynonymsLoxistatin, (2S,3S)-trans-Epoxysuccinyl-L-leucylamido-3-methylbutane Ethyl Ester, E-64d
    DescriptionA cell-permeable, irreversible inhibitor of cysteine proteases. Similar to E-64 (Cat. No. 324890) but is devoid of charged groups. The inhibitory activity of EST has been attributed to E-64c, the free acid formed by hydrolysis of the ester in vivo. Used in animal models of muscular dystrophy. Recommended working concentration is 10-100 µM.
    FormWhite solid
    CAS number88321-09-9
    RTECSRR0404300
    Chemical formulaC₁₇H₃₀N₂O₅
    Structure formulaStructure formula
    Purity≥97% by HPLC
    SolubilityDMSO
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 6 months at -20°C.
    Toxicity Irritant
    ReferencesInomata, M., et al. 1996. Arch. Biochem. Biophys. 328, 129.
    Mehdi, S. 1991. Trends Biochem. Sci. 16, 150.
    McGowan, E.B., et al. 1989. Biochem. Biophys. Res. Commun. 158, 432.
    Tamai, M., et al. 1987. J. Pharmacobiodyn. 10, 678.
    Komatsu, K., et al. 1986. Exp. Neurol. 91, 23.
    Tamai, M., et al. 1986. J. Pharmacobiodyn. 9, 672.
    Citation
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.