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341220 Exo1 - CAS 75541-83-2 - Calbiochem

341220
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₅H₁₂FNO₃75541-83-2

Products

Catalogue NumberPackaging Qty/Pack
341220-25MG Plastic ampoule 25 mg
Description
OverviewA cell-permeable methylanthranilate analog that reversibly inhibits vesicular traffic from ER to Golgi in mammalian cells (IC50 = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and redirecting the traffic back to ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor Brefeldin A (BFA; Cat. No. 203729), its effect seems to be limited to Golgi, as no effect can be detected on endocytic organelles, such as endosomes and TGN (trans-Golgi network). Its inhibitory mechanism and target(s) appear to be quite different from those of BFA and thus serves as a valuable alternative that complements BFA in membrane traffic studies.
Catalogue Number341220
Brand Family Calbiochem®
Synonyms2-(4-Fluorobenzoylamino)methylbenzoate, 2-(4-Fluorobenzoylamino)benzoic Acid Methyl Ester, ER Export Inhibitor I
References
ReferencesFeng. Y., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 6469.
Product Information
CAS number75541-83-2
ATP CompetitiveN
FormWhite solid
Hill FormulaC₁₅H₁₂FNO₃
Chemical formulaC₁₅H₁₂FNO₃
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetGolgi ARF1 GTPase
Primary Target IC<sub>50</sub>20 µM inhibiting vesicular traffic from ER to Golgi in BSC1 cells
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Exo1 - CAS 75541-83-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Exo1 - CAS 75541-83-2 - Calbiochem Certificates of Analysis

TitleLot Number
341220

References

Reference overview
Feng. Y., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 6469.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-April-2008 RFH
Synonyms2-(4-Fluorobenzoylamino)methylbenzoate, 2-(4-Fluorobenzoylamino)benzoic Acid Methyl Ester, ER Export Inhibitor I
DescriptionA cell-permeable methylanthranilate analog that reversibly blocks vesicular traffic from the ER to the Golgi in mammalian cells (IC50 = 20 µM in BSC1 cells) by inducing tubulation and collapsing of the Golgi membrane and re-directing the traffic back to the ER. Acts as a potent and selective modifier of Golgi ARF1 GTPase activity. Unlike another membrane traffic inhibitor, Brefeldin A (BFA) (Cat. No. 203729), the effects appear to be limited to the Golgi, as no effects are detected on endocytic organelles, such as endosomes and trans-Golgi network (TGN). The inhibitory mechanism and target(s) appear to be different from those of BFA, thus making it a valuable alternative that complements BFA in membrane trafficking studies.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number75541-83-2
Chemical formulaC₁₅H₁₂FNO₃
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (25 mg/ml), DMF (25 mg/ml), Methanol (5 mg/ml), or Ethanol (5 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
ReferencesFeng. Y., et al. 2003. Proc. Natl. Acad. Sci. USA 100, 6469.