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341583 FGF-2, Basic, Bovine

341583
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
341583-25UG Plastic ampoule 25 μg
Description
OverviewNative, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells.
Catalogue Number341583
Brand Family Calbiochem®
SynonymsnbbFGF, Fibroblast Growth Factor, Basic, Bovine
References
ReferencesOhmiya, M., et al. 2001. J. Neurosci. Res. 65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol. 147, 120.
Product Information
CAS number106096-93-9
FormLyophilized
FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <0.1 ng/µg FGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSLK3641300
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

FGF-2, Basic, Bovine SDS

Title

Safety Data Sheet (SDS) 

FGF-2, Basic, Bovine Certificates of Analysis

TitleLot Number
341583

References

Reference overview
Ohmiya, M., et al. 2001. J. Neurosci. Res. 65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol. 147, 120.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-May-2008 RFH
SynonymsnbbFGF, Fibroblast Growth Factor, Basic, Bovine
DescriptionNative, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. A multifunctional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Known to activate phopholipase D in endothelial cells. Higher concentrations are found in the brain and pituitary gland. Purified from bovine brain by sequential chromatography.
FormLyophilized
FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.
CAS number106096-93-9
RTECSLK3641300
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: <0.1 ng/µg FGF
Biological activityED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts
SolubilityReconstitute to a concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 4072
ReferencesOhmiya, M., et al. 2001. J. Neurosci. Res. 65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol. 147, 120.