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341262 Factor B, Human

341262
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
341262-250UG Plastic ampoule 250 μg
Description
OverviewNative factor B from human serum. Single-chain glycoprotein present in normal human serum at about 200 µg/ml. During activation of complement via the alternative pathway, Factor B is cleaved and thus activated by Factor D into two fragments of M.W. of 30 kDa (Ba) and 63 kDa (Bb). The Bb fragment contains the serine protease enzymatic active site. The activated Bb fragment continues activation via the alternative pathway by cleaving, and thus activating, C3 and C5.
Catalogue Number341262
Brand Family Calbiochem®
SynonymsC3 Proactivator
References
ReferencesKam, C.M., et al. 1987. J. Biol. Chem. 262, 3444.
Gotze, O. and Muller-Eberhard, H.J. 1971. J. Exp. Med. 134, 90s.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Applications
Biological Information
Biological activity≥1,200 Factor BH50 units/mg; ≥50% of Factor B activity in NHS (mg/mg basis)
Purity≥90% by SDS-PAGE
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Physicochemical Information
ContaminantsIgG, IgA, IgM, C3, C4, Factor H, Factor I, or albumin (Ouchterlony): ≤trace amounts
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Factor B, Human Certificates of Analysis

TitleLot Number
341262

References

Reference overview
Kam, C.M., et al. 1987. J. Biol. Chem. 262, 3444.
Gotze, O. and Muller-Eberhard, H.J. 1971. J. Exp. Med. 134, 90s.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-June-2008 RFH
SynonymsC3 Proactivator
DescriptionNative factor B from human serum. Single-chain glycoprotein present in normal human serum at about 200 µg/ml. During activation of complement via the alternative pathway, Factor B is cleaved, and thus activated by Factor D into two fragments of M.W. of ~30 kDa (Ba) and ~63 kDa (Bb). The Bb fragment contains the serine protease enzymatic active site. The activated Bb fragment continues activation via the alternative pathway by cleaving, and thus activating, C3 and C5.
FormLiquid
FormulationIn PBS, pH 7.2.
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Purity≥90% by SDS-PAGE
ContaminantsIgG, IgA, IgM, C3, C4, Factor H, Factor I, or albumin (Ouchterlony): ≤trace amounts
Biological activity≥1,200 Factor BH50 units/mg; ≥50% of Factor B activity in NHS (mg/mg basis)
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesKam, C.M., et al. 1987. J. Biol. Chem. 262, 3444.
Gotze, O. and Muller-Eberhard, H.J. 1971. J. Exp. Med. 134, 90s.