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341668 Fibronectin, Rat Plasma

341668
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
341668-500UG Plastic ampoule 500 μg
Description
OverviewNative fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Catalogue Number341668
Brand Family Calbiochem®
References
Product Information
CAS number86088-83-7
FormLiquid
FormulationIn 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.
Quality LevelMQ100
Applications
Biological Information
Puritysingle major band by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsNone detected by IEP using anti-fibronectin and anti-rat serum
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Fibronectin, Rat Plasma SDS

Title

Safety Data Sheet (SDS) 

Fibronectin, Rat Plasma Certificates of Analysis

TitleLot Number
341668
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-May-2008 RFH
DescriptionNative fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
FormLiquid
FormulationIn 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditionsProcedure for Coating of Tissue Culture Plates with Fibronectin This procedure is based on the use of 21 cm2 dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and the 1 mg package size of fibronectin. One mg of fibronectin is sufficient to coat 10 dishes at 5 µg/cm2. 1. After thawing the fibronectin solution as indicated above, bring the solution to a final volume of 20 ml with sterile serum-free medium that has been preheated to 37°C. This yields a fibronectin solution of 50 µg/ml. 2. Add 2 ml of this solution to each of 10 tissue culture plates and swirl gently to completely coat the entire growth surface. 3. Allow dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces. 4. Tilt each dish and remove the fibronectin solution using a sterile pipette. Do not permit the pipette tip to disturb the growth surface. 5. Add cell suspension in medium directly to the fibronectin-coated dishes. This procedure is meant to serve as a guideline and optimal may require optimization.
CAS number86088-83-7
Puritysingle major band by SDS-PAGE
ContaminantsNone detected by IEP using anti-fibronectin and anti-rat serum
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
Toxicity Standard Handling
Merck USA index14, 4075