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344289 Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum

344289
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
344289-1SET Glass bottle 1 set
Description
OverviewContains 10 vials per set, each containing 10 ml of Freund's complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.
Catalogue Number344289
Brand Family Calbiochem®
References
Product Information
CAS number9007-81-2
FormClear amber to slightly cloudy liquid
FormulationYellow, oily, bacterial suspension.
Quality LevelMQ100
Applications
ApplicationFreund’s Complete Adjuvant, Modified, Mycobacterium butyricum, CAS 9007-81-2, provided as a set of 10 vials each containing: 85% Drakeol 5NF, 15% Arlacel A, and 0.1% M.butyricum dry cells.
Application NotesImmunization
Application CommentsComposition
The Freund's Adjuvant set contains 10 identical ampules, each with 10 ml of Freund's Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells

Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSLS6860000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum SDS

Title

Safety Data Sheet (SDS) 

Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum Certificates of Analysis

TitleLot Number
344289
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-August-2007 RFH
ApplicationImmunization
Description1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.
BackgroundThe word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
FormClear amber to slightly cloudy liquid
FormulationYellow, oily, bacterial suspension.
CAS number9007-81-2
RTECSLS6860000
CommentsComposition
The Freund's Adjuvant set contains 10 identical ampules, each with 10 ml of Freund's Complete Adjuvant homogenized in the following preparations by weight:
85% Drakeol 5 NF
15% Arlacel A, mannide monooleate emulsifier
0.1% Mycobacterium butyricum dry cells

Sample Preparation
1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.
2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.
3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling