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345810 G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

345810
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₂₀H₄₀N₄O₁₀ · 2H₂SO₄108321-42-2

Products

Catalogue NumberPackaging Qty/Pack
345810-100GM Glass bottle 100 gm
345810-1GM Glass bottle 1 gm
345810-250MG Plastic ampoule 250 mg
345810-25GM Plastic ampoule 25 gm
345810-500MG Plastic ampoule 500 mg
345810-5GM Glass bottle 5 gm
Description
OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).
Catalogue Number345810
Brand Family Calbiochem®
SynonymsG418, G 418, NMD Inhibitor I
References
ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.
Product Information
CAS number108321-42-2
FormWhite powder
Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Biological activityAssay: ≥730 µg/mg
Purity≥98% by TLC
Physicochemical Information
ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSCB9378500
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

TitleLot Number
345810

References

Reference overview
Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.

Brochure

Title
Antibiotics Profiler
Bulk Product Guide
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title
  • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
  • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
  • Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision01-December-2011 JSW
    SynonymsG418, G 418, NMD Inhibitor I
    DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
    FormWhite powder
    Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
    CAS number108321-42-2
    RTECSCB9378500
    Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
    Structure formulaStructure formula
    Purity≥98% by TLC
    ContaminantsAmmonia: ≤1.0%; heavy metals: ≤100 ppm
    Biological activityAssay: ≥730 µg/mg
    SolubilitySolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
    Storage +15°C to +30°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.
    Toxicity Standard Handling
    ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
    Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
    Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
    Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
    Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
    Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
    Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
    Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
    Jimenez, A., and Davies, J. 1980. Nature 287, 869.
    Citation
  • Ellen F. Cohn, et al. (2006) Abrupt Expression of TLR4 in TLR4-Deficient Macrophages Imposes a Selective Disadvantage: Genetic Evidence for TLR4-Dependent Responses to Endogenous, Nonmicrobial Stimuli. Journal of Immunology 176, 1185-1194.
  • Aarti N. Desai, et al. (2006) Involvement of GRK3 and GRK2 in Down-regulation of the {alpha}2B-Adrenoceptor. Journal of Pharmacology and Experimental Therapeutics 317, 1027-1035.
  • Related Products & Applications

    Alternative Products

    Catalogue Number Description
    345812 G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

    Categories

    Life Science Research > Inhibitors and Biochemicals > Small Molecules & Inhibitors > Other Inhibitors of Biological Interest > Protein Synthesis Inhibitors
    Life Science Research > Inhibitors and Biochemicals > Biochemicals > Antibiotics > Antimicrobial Agents
    Life Science Research > Cell Culture and Systems > Antibiotics & Selection Agents