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361524 GSK-3β, His•Tag®, Human, Recombinant, E. coli

361524
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
361524-100UG Plastic ampoule 100 μg
Description
OverviewRecombinant, human GSK-3β fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. A dual specificity kinase that plays important roles in insulin- and Wnt-mediated cellular signalings. In AD (Alzheimer’s disease), GSK3β has a major role in microtubule stability by its ability to phosphorylate the microtubule associated protein tau. Its known substrates include glycogen synthase, p90rsk, Tau, c-Jun, and CREB. MW: 53,000-56,000.
Catalogue Number361524
Brand Family Calbiochem®
SynonymsGSK-3β, Tau Protein Kinase I, TPK I
References
ReferencesHarwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.
Product Information
FormSolid
FormulationLyophilized from 0.1 M NH₄CO₃.
Quality LevelMQ100
Applications
ApplicationGSK-3β, His-Tag®, Human, Recombinant, E. coli, is a dual specificity kinase that plays important roles in insulin- and Wnt-mediated signaling. It is inactivated by phosphorylation at Ser9.
Biological Information
Biological activity1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

GSK-3β, His•Tag®, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

GSK-3β, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
361524

References

Reference overview
Harwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-January-2009 JSW
SynonymsGSK-3β, Tau Protein Kinase I, TPK I
DescriptionRecombinant, human GSK-3β fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that was first characterized by its role in intermediary metabolism. GSK-3β is a dual-specificity kinase that is important role in insulin- and Wnt-mediated cellular signaling events. In Alzheimer's disease GSK-3β is reported to affect microtubule stability via the phosphorylation of microtubule associated protein, Tau. Other known substrates include glycogen synthase, p90rsk, c-Jun, and CREB. MW: 53,000-56,000
FormSolid
FormulationLyophilized from 0.1 M NH₄CO₃.
Recommended reaction conditions25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 12 mM MgCl2, 2 mM DTT, 100 µM Na3VO4, 200 µM ATP, 30°C.
Purity≥90% by SDS-PAGE
Biological activity1 µg GSK-3β maximally phosphorylates 100 ng Tau protein in 30 min in an in vitro kinase assay at 30°C, pH 7.5
SolubilityReconstitute in sterile PBS to a final concentration of 1 mg/ml.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C.
Toxicity Standard Handling
ReferencesHarwood, A., and Braga, V.M. 2003. Nat. Cell Biol. 5, 275.
Bhat, R.V., and Budd, S.L. 2002. Neurosignals 11, 251.