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354406 Glutaredoxin-S2, E. coli

354406
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
354406-1MG Plastic ampoule 1 mg
Description
OverviewNative glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
Catalogue Number354406
Brand Family Calbiochem®
SynonymsGrx1
References
ReferencesRuoppolo, M., et al. 1997. Biochemistry 36, 12259.
Sun, C., et al. 1997. Protein Sci. 6, 383.
Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
Hoog, J.O., et al. 1986. Gene 43, 13.
Product Information
Unit of DefinitionUnits are defined using a standard HED assay.
FormLyophilized
FormulationLyophilized from 0.5% NH₄HCO₃.
Quality LevelMQ100
Applications
ApplicationGlutaredoxin-S2, E. coli, acts as a glutathione-dependent hydrogen donor for ribonucleotide reductase. Useful as a general disulfide reductant for the in vitro study of protein folding mechanisms.
Biological Information
Specific Activity≥175 µmole NADPH oxidized/min/mg
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Glutaredoxin-S2, E. coli SDS

Title

Safety Data Sheet (SDS) 

Glutaredoxin-S2, E. coli Certificates of Analysis

TitleLot Number
354406

References

Reference overview
Ruoppolo, M., et al. 1997. Biochemistry 36, 12259.
Sun, C., et al. 1997. Protein Sci. 6, 383.
Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
Hoog, J.O., et al. 1986. Gene 43, 13.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-July-2008 RFH
SynonymsGrx1
DescriptionNative glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
FormLyophilized
FormulationLyophilized from 0.5% NH₄HCO₃.
Specific activity≥175 µmole NADPH oxidized/min/mg
Unit definitionUnits are defined using a standard HED assay.
SolubilityReconstitute in 1 ml 50 mM Tris-HCl, 1 mM EDTA, pH 7.5 to yield a final concentration of 1 mg/ml.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
Toxicity Standard Handling
ReferencesRuoppolo, M., et al. 1997. Biochemistry 36, 12259.
Sun, C., et al. 1997. Protein Sci. 6, 383.
Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
Hoog, J.O., et al. 1986. Gene 43, 13.