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69049 Pellet Paint® Co-Precipitant

69049
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69049-3 Plastic ampoule 125 rxn
69049-4 Plastic ampoule 1000 rxn
Description
OverviewPellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (McCormick 1995, McCormick 1996). The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.

Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated (Taggart 1998). Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.

Pellet Paint Co-Precipitant is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package) can be used as a correction factor when determining nucleic acid concentration (McCormick 1996). Pellet Paint Co-Precipitant is compatible with automatted Cy5® sequencers. Pellet Paint NF Co- Precipitant is recommended for use with PE Applied Biosystems automated sequencers.



Catalogue Number69049
Brand Family Novagen®
Features and benefits• Direct visualization and tracking of precipitated material
References
ReferencesMcCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.
Product Information
250 µl or 2 mlPellet Paint Co-Precipitant
1 ml or 8 ml3 M Sodium Acetate, pH 5.2
DeclarationThis product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates
FormLiquid
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Pellet Paint® Co-Precipitant SDS

Title

Safety Data Sheet (SDS) 

Pellet Paint® Co-Precipitant Certificates of Analysis

TitleLot Number
69049

References

Reference overview
McCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.

Citations

Title
  • Brooks, C.L., 2007. Journal of Biological Chemistry 282, 22804.
  • Buttner, M.P., et al. 2007. Applied and Enviornmental Microbiology 73, 3505.
  • Halling, R.E., et al. 2007. Mycologia 99, 310.
  • Quan, S., et al. 2007. Journal of Biological Chemistry 282, 28823.
  • Tsai, C.J., et al. 2007. Procedings of the National Academy of Science 104, 7851.
  • Nowak, J.E., et al. 2006. Bio/Techniques 40, 220.
  • Lee, Y., et al. 2005 Clinical Cancer Research 11, 1059.
  • Mori, T., et al. 2005. Journal of Clinical Oncology 23, 9351.
  • Pendleton, L.C., et al. 2005. Journal of Biological Chemistry 280, 24252.
  • Beischlag, T.V., et al. 2004. Journal of Biological Chemistry 279, 54620.
  • Xu, M., et al. 2002. Journal of Clinical Microbiology 40, 2626.
  • Taggart, E.W., et al. 1998. Journal of Clinical Microbiology 36, 3408.
  • User Protocols

    Title
    TB146 Pellet Paint® Co-Precipitant