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361527 PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

361527
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
361527-1SET Plastic ampoule 1 set
Description
OverviewRecognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
Catalogue Number361527
Brand Family Calbiochem®
References
Product Information
FormLiquid
Formulation100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
Positive controlPervanadate-treated HepG2 (included)
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
Application CommentsFor immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
ImmunogenHuman
Clone2D3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3) SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3) Certificates of Analysis

TitleLot Number
361527
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-September-2009 RFH
ApplicationELISA (0.05 µg/ml)
Immunoblotting (0.5 µg/ml)
DescriptionMouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
Clone2D3
IsotypeIgG₁
Specieshuman, mouse
Positive controlPervanadate-treated HepG2 (included)
FormLiquid
Formulation100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
Preservative≤0.1% sodium azide (antibody only)
CommentsFor immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
Toxicity Standard Handling