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346104 Plk2, GST- Fusion, Human, Recombinant, S. frugiperda

346104
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
346104-10UG Plastic ampoule 10 μg
Description
OverviewFull length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening.
Catalogue Number346104
Brand Family Calbiochem®
SynonymsPolo-like kinase 2, Human, Recombinant
Application Data
Phosphorylation of SER/THR 16 substrate was measured in the presence of increasing concentration of Plk2 as outlined in the Recommended Reaction Conditions above.
References
ReferencesEckerdt, F., et al. 2005. Oncogene 24, 267.
Warnke, S., et al. 2004.Curr Biol. 14, 1200.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5.
FormLiquid
FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥70% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
S PhraseS: 26-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Plk2, GST- Fusion, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

Plk2, GST- Fusion, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
346104

References

Reference overview
Eckerdt, F., et al. 2005. Oncogene 24, 267.
Warnke, S., et al. 2004.Curr Biol. 14, 1200.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2008 JSW
SynonymsPolo-like kinase 2, Human, Recombinant
Application Data
Phosphorylation of SER/THR 16 substrate was measured in the presence of increasing concentration of Plk2 as outlined in the Recommended Reaction Conditions above.
DescriptionFull length, recombinant, human Plk2 fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. Plk2 is a G1 specific kinase that is specifically detected in brain, heart, and lung. Plk2 expression increases in the presence of DNA-damaging agents. The active kinase is useful for the study of Plk2 regulation and inhibitor screening.
FormLiquid
FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 detergent, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
50 mM HEPES, 10 mM MgCl2, 1 mM EGTA, 0.01% Brij®-35 detergent, 200 µM ATP, 2 µM SER/THR 16 substrate; incubate at room temperature for 1 h.
Purity≥70% by SDS-PAGE
Unit definitionOne unit is defined as the amount of enzyme that will phosphorylate 1 pmol SER/THR 16 substrate per min at 30°C, pH 7.5.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesEckerdt, F., et al. 2005. Oncogene 24, 267.
Warnke, S., et al. 2004.Curr Biol. 14, 1200.