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189738 Streptavidin, TEXAS RED® Conjugate

189738
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Products

Catalogue NumberPackaging Qty/Pack
189738-1ML Glass bottle 1 ml
Description
Catalogue Number189738
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunohistochemistry
Application NotesELISA (1:2000-1:5000)
Flow Cytometry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Application CommentsBiotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 106 cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Yes
Special InstructionsDo not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Streptavidin, TEXAS RED® Conjugate Certificates of Analysis

TitleLot Number
189738
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-June-2007 RFH
ApplicationELISA (1:2000-1:5000)
Flow Cytometry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
DescriptionStreptavidin conjugated to TEXAS RED® fluorescent dye. Specific for biotin, biotinylated proteins, or biotinylated antibodies.
FormLiquid
FormulationIn 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsBiotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 106 cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Protect from light
Do Not Freeze Yes
Special InstructionsDo not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.
Toxicity Standard Handling