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164610 p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem

164610
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₈H₉HgNO₂6283-24-5

Products

Catalogue NumberPackaging Qty/Pack
164610-1SET Fibre case 1 set
164610-700MG Plastic Bag(s) 700 mg
Description
OverviewAn organomercurial agent used for the activation of latent MMPs in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
Note: 1 set = 5 x 700 mg.
Catalogue Number164610
Brand Family Calbiochem®
SynonymsAPMA
References
ReferencesChen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
Stricklin, G.P., et al. 1983. Biochemistry 22, 61.
Product Information
CAS number6283-24-5
ATP CompetitiveN
FormYellow solid
Hill FormulaC₈H₉HgNO₂
Chemical formulaC₈H₉HgNO₂
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetActivation of latent MMPs in vitro
Purity≥90% by titration
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSOV5550000
Safety Information
R PhraseR: 26/27/28-36/37/38

Very toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem Certificates of Analysis

TitleLot Number
164610

References

Reference overview
Chen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
Stricklin, G.P., et al. 1983. Biochemistry 22, 61.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-September-2007 RFH
SynonymsAPMA
DescriptionAn organomercurial agent used for the activation of latent matrix metalloproteinases (MMPs) in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
FormYellow solid
Recommended reaction conditionsThe MMPs are members of a family of at least twenty Zn2+-dependent endopeptidases that function extracellularly at neutral pH. The MMPs are initially synthesized as latent or inactive zymogens that contain a regulatory propeptide. This propeptide confers the latency by binding to the Zn2+ at the enzyme's active site. MMP activation can be achieved by autoactivation and by the action of other MMPs or proteases, such as furin, urokinase, plasmin, and trypsin, as well as by organomercurial compounds. The following is a protocol from Stricklin, et al. (1983. Biochemistry 22, 61) describing the use of paminophenylmercuric acetate (APMA) to activate pro-MMPs. This protocol is good for other types of organomercurials such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), and mersalyl. Organomercurial stock solutions Prepare stock solutions in 0.1 M NaOH at a concentration range of 10-50 mM just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl. (e.g. Marcy, A.I. et al. 1991. Biochemistry 30, 6476). ProMMPs Prepare proenzyme solutions in 0.1 M Tris-HCl, pH 7.5 at 1-2 mg/ml. Activation of proMMPs To initiate activation, combine proMMP solution with organomercurial stock in a 10:1 volume* ratio (MMPs to organomercurial). Incubate the mixture at 37°C for 2-3 hrs**. *When more organomercurial is desired, make the stock solution more concentrated. Do not exceed the above ratio so that the volume of organomercurial in the mixture is more than about 1/10 of that of the proMMP solution; this could result in significant changes in pH. **It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with fixed concentration of proMMP and organomercurial would be incubated as described above. Remove aliquots of sample at various time points. Stop the reaction by the addition of concentrated SDS-sample buffer (for example, add 10 µl of 2X sample buffer to a 10 µl aliquot or add 2 µl of 5X sample buffer to an 8 µl aliquot) and then boil. The progress of the activation can then be monitored qualitatively by analyzing these time aliquots on a 12% SDS-PAGE gel. Removing organomercurial from the mixture after activation The activated MMPs can be used without the removal of the organomercurial. Researchers are referred to the following reference for the removal of organomercurial by gel filtration if desired: Marcy, A.I., et al. 1991. Biochemistry 30, 6476
CAS number6283-24-5
RTECSOV5550000
Chemical formulaC₈H₉HgNO₂
Structure formulaStructure formula
Purity≥90% by titration
SolubilityDMSO (10 mg/ml) or 0.1 N NaOH (10 mg/ml)
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
Toxicity Highly Toxic
ReferencesChen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
Stricklin, G.P., et al. 1983. Biochemistry 22, 61.