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Merck

PICM0RG50

Millicell® 6 well standing inserts

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, sterile

Synonym(s):

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm

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About This Item

UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32011202

Product Name

Millicell® 6 well standing inserts, pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, sterile

material

transparent PTFE membrane
polystyrene housing (5 mm height)

sterility

ethylene oxide treated
sterile

feature

hydrophilic

manufacturer/tradename

Millicell®

packaging

pkg of Individually blister packaged

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

5 mm

diam.

30 mm

size

6 wells

surface area

4.2 cm2

Quality Level

400

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

Related Categories

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany

Application

  • Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry
  • Biopore Membrane (hydrophilic PTFE) is ideal for low protein-binding, live cell viewing, and immunofluorescent applications

Features and Benefits

  • For high cell viability and superior study of three dimensional explant structure
  • Lower height allows them to fit inside a standard petri dish
  • The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
  • The membrane is optically clear and optimized for long-term organotypic explant maintenance

General description

With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.

Preparation Note

To ensure proper wetting of the membrane from the basolateral side and to mitigate a chance of bubble formation:
  1. Add assay solution or media to the receiver well: 2 mL for 6 well format orequivalent dimensions.
  2. With forceps, place the insert into the receiver well at a 30°-45° angle togradually wet it out from the basolateral side.
  3. Once edges start to wet out, gently lower the insert until it is fully seatedinto the well.

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic
Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks
Abigail K Myers et al.
Development (Cambridge, England), 147(2) (2020-01-10)
The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
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Global Trade Item Number

SKUGTIN
PICM0RG5004053252360763

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