128136 7α, 25-dihydroxycholesterol - Calbiochem

128136
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₇H₄₆O₃

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128136-1MG
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      Glass bottle 1 mg
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      Description
      OverviewA highly potent and specific oxysterol EBI2 (GPR183) agonist with a kd of 450 pM in a saturation binding analysis, and activates EBI2 (EC50=140 pM compared with 2.1nM for its enantiomer 7b,25-OHC) in a GTP-γ assay. Dose-dependenty suppresses forskolin-induced cAMP accumulation in EBI2-expressing SK-N-MC/CRE-β galactosidase cell line with an IC50 of 2 nM, but not in control cells. Stimulates migration of LPS-activated spleen B cells and T cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells from EBI2-deficient mice.
      Catalogue Number128136
      Brand Family Calbiochem®
      SynonymsEBI2 agonist, EBI2 Activator, GPR183 Activator, 7alpha,25-OHC
      References
      ReferencesLiu, C., et al. 2011. Nature 475, 519.
      Product Information
      FormWhite powder
      Hill FormulaC₂₇H₄₆O₃
      Chemical formulaC₂₇H₄₆O₃
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      7α, 25-dihydroxycholesterol - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      7α, 25-dihydroxycholesterol - Calbiochem Certificates of Analysis

      TitleLot Number
      128136

      References

      Reference overview
      Liu, C., et al. 2011. Nature 475, 519.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-February-2013
      SynonymsEBI2 agonist, EBI2 Activator, GPR183 Activator, 7alpha,25-OHC
      DescriptionA highly potent and specific oxysterol EBI2 (GPR183) agonist with a kd of 450 pM in a saturation binding analysis, and activates EBI2 (EC50=140 pM compared with 2.1nM for its enantiomer 7b,25-OHC) in a GTP-γ assay. Dose-dependenty suppresses forskolin-induced cAMP accumulation in EBI2-expressing SK-N-MC/CRE-β galactosidase cell line with an IC50 of 2 nM, but not in control cells. Stimulates migration of LPS-activated spleen B cells and T cells in a dose-dependent manner. In addition, pharmacological inhibition of its biosynthesis in vivo by Clotrimazole, a CYP7B1inhibitor promotes the migration of adoptively transferred pre-activated B cells to the T/B boundary, mimicking the phenotype of pre-activated B cells from EBI2-deficient mice.
      FormWhite powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₇H₄₆O₃
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (25 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesLiu, C., et al. 2011. Nature 475, 519.