178375 APO-10 - CAS 2190-95-6 - Calbiochem

178375
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₂H₂₇PO2190-95-6

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      Plastic ampoule 1 gm
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      Description
      OverviewNovel non-ionic detergent useful for plasmid DNA isolation. Plasmid DNA isolated using APO-10 does not require the use of phenol/chloroform or proprietary resins. Provides excellent yields of high quality DNA. Allows plasmid DNA to be sequenced through difficult regions without the need to subclone into single-stranded vectors.
      Catalogue Number178375
      Brand Family Calbiochem®
      SynonymsDimethyldecylphosphine Oxide
      References
      ReferencesLi, L., et al. 2003. Hypertension 42, 997.
      Altschuler, M., et al. 1994. Biotechniques 17, 434.
      Product Information
      CAS number2190-95-6
      Aggregation Number131
      CMC4.6 mM
      FormWhite solid
      Hill FormulaC₁₂H₂₇PO
      Chemical formulaC₁₂H₂₇PO
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      APO-10 - CAS 2190-95-6 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      APO-10 - CAS 2190-95-6 - Calbiochem Certificates of Analysis

      TitleLot Number
      178375

      References

      Reference overview
      Li, L., et al. 2003. Hypertension 42, 997.
      Altschuler, M., et al. 1994. Biotechniques 17, 434.

      Brochure

      Title
      Biological Detergents Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-April-2008 RFH
      SynonymsDimethyldecylphosphine Oxide
      DescriptionNovel non-ionic detergent useful for plasmid DNA isolation (aggregation number: 131; CMC: 4.6 mM). Plasmid DNA isolated using APO-10 does not require the use of phenol/chloroform or proprietary resins. Provides excellent yields of high quality DNA. Allows plasmid DNA to be sequenced through difficult regions without the need to subclone into single-stranded vectors.
      FormWhite solid
      Recommended reaction conditions
      Protocol for Plasmid DNA Isolation using APO-10 1. Centrifuge 1.5 ml of bacterial culture at 12,000 x g for 2 min. 2. Resuspend the pellet in 100 µl of 50 mM glucose, 25 mM Tris-HCl, 10 mM EDTA, 10 µg/ml RNase, pH 8.0. 3. Add 200 µl of 200 mM NaOH and 1% APO-10. Mix gently by inverting. Incubate at room temperature for 5 min. 4. Add 150 µl of 3 M potassium acetate buffer, pH 4.8. Mix gently by inverting. Incubate at room temperature for 5 min. 5. Centrifuge sample for 15 min at 4°C. Collect the supernatant. 6. Add 1 ml of 100% ethanol to the supernatant, mix, and centrifuge for 15 min at 4°C. Wash the pellet in 70% ethanol and dry. 7. The DNA pellet can be resuspended in 25 µl TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA). RNase A can be added to digest any RNA.
      CAS number2190-95-6
      Chemical formulaC₁₂H₂₇PO
      Structure formulaStructure formula
      SolubilityH₂O (10 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.
      Toxicity Irritant
      ReferencesLi, L., et al. 2003. Hypertension 42, 997.
      Altschuler, M., et al. 1994. Biotechniques 17, 434.