164599 Aminopeptidase N, Rat

164599
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164599-10U
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      Plastic ampoule 10 u
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      Description
      OverviewNative aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that, functions with broad specificity to remove N-terminal amino acids from peptides. It has been reported that levels of aminopeptidase N are elevated in cancer subjects and correlate with tumor load, and thus, the enzyme is believed to play a role in angiogenesis.
      Catalogue Number164599
      Brand Family Calbiochem®
      SynonymsAPN/CD13, aminopeptidase N
      References
      ReferencesBhagwat, S.V., et al. 2003. Blood 101, 1818.
      van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
      Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-<i>p</i>NA per min at 37°C, pH 7.2
      EC number3.4.11.2
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥100 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Aminopeptidase N, Rat SDS

      Title

      Safety Data Sheet (SDS) 

      Aminopeptidase N, Rat Certificates of Analysis

      TitleLot Number
      164599

      References

      Reference overview
      Bhagwat, S.V., et al. 2003. Blood 101, 1818.
      van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
      Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-April-2008 RFH
      SynonymsAPN/CD13, aminopeptidase N
      DescriptionNative aminopeptidase N purified from rat renal brush-border membranes and cleaved with papain to produce soluble enzyme. A single-chain, integral membrane protease that functions to remove N-terminal amino acids from peptides with broad specificity. Aminopeptidase N is believed to play a role in angiogenesis and elevated levels of aminopeptidase N have been reported in patients with various types of cancer and have been correlated with tumor load. Also involved in the final stages of digestion of small peptides in the intestinal brush border.
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCl, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions200 mM Tris-HCl, pH 7.2, 100 µl substrate (L-leu-pNA; final concentration 1 mM) in 200 mM Tris-HCl, pH 7.2, enzyme sample, final volume 200 µl; incubate at 37°C for 10 min.*; cleavage is monitored at 405 nm. *Note: Incubation time of 10 min. is provided as a guideline. The incubation time depends on the activity of the enzyme and the sensitivity of the instrument and should be determined for individual assays.
      EC number3.4.11.2
      Purity≥90% by SDS-PAGE
      Specific activity≥100 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol L-Leu-pNA per min at 37°C, pH 7.2
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBhagwat, S.V., et al. 2003. Blood 101, 1818.
      van Hensbergen, Y., et al. 2002. Clin. Cancer Res. 8, 3747.
      Ishii, K., et al. 2001. Biol. Pharm. Bull. 24, 226.