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CB1050 Anti-CD99 Mouse mAb (3A10)

Anti-CD99, mouse monoclonal, clone 3A10, recognizes the ~25 kDa CD99 in K562 cells. It is validated for ELISA and Western blotting.

Anti-CD99 Mouse mAb (3A10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~25 kDa CD99 protein in K562 cells.
Catalogue NumberCB1050
Brand Family Calbiochem®
Application Data
Detection of human CD99 by immunoblotting. Sample: Whole cell lysate from K562 cells. Primary antibody: Anti-CD99 Mouse mAb (3A10) (Cat. No. CB1050) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlK562 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 23-123 human CD99, expressed as GST fusion protein
Clone3A10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1050 0

Documentation

Anti-CD99 Mouse mAb (3A10) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human CD99 by immunoblotting. Sample: Whole cell lysate from K562 cells. Primary antibody: Anti-CD99 Mouse mAb (3A10) (Cat. No. CB1050) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~25 kDa CD99 protein.
BackgroundThis protein is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 23-123 human CD99, expressed as GST fusion protein
Clone3A10
IsotypeIgG2a
Specieshuman
Positive controlK562 cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review