208730 Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3)

208730
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, HMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
208730-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2). Recognizes both native and denatured forms of the small subunit of calpain.
      Catalogue Number208730
      Brand Family Calbiochem®
      Application Data
      This antibody can be used to immunoprecipitate the small subunit of Calpain I and Calpain II under native and denatured conditions. Immunoprecipitation (10% SDS-PAGE) Samples: A431 cells lysed under native conditions and immunoprecipitated with clone 28F3 anti-calpain I,II small subunit (Cat. No. 208730) (lane 1), an irrelevant antibody (lane 2) and direct lysate (lane 3). Primary antibody concentration: 0.5 µg/ml (Cat. No: 208730) Secondary antibody concentration: 0.2 µg/ml goat anti-mouse IgG-HRP, γ-chain specific (Cat. No. 401207) Exposure: 1 min Obtained results: Bands at ~30 kDa in lanes 1 and 3
      References
      Product Information
      FormLiquid
      FormulationIn 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
      Positive controlMDBK cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (1:2000)
      Immunoblotting (1:2000)
      Immunoprecipitation (1:500)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified calpain from human placenta
      ImmunogenHuman Placenta
      Clone28F3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Bovine
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calpain-1/2, Small Subunit Mouse mAb (28F3) Certificates of Analysis

      TitleLot Number
      208730
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      ApplicationELISA (1:2000)
      Immunoblotting (1:2000)
      Immunoprecipitation (1:500)
      Application Data
      This antibody can be used to immunoprecipitate the small subunit of Calpain I and Calpain II under native and denatured conditions. Immunoprecipitation (10% SDS-PAGE) Samples: A431 cells lysed under native conditions and immunoprecipitated with clone 28F3 anti-calpain I,II small subunit (Cat. No. 208730) (lane 1), an irrelevant antibody (lane 2) and direct lysate (lane 3). Primary antibody concentration: 0.5 µg/ml (Cat. No: 208730) Secondary antibody concentration: 0.2 µg/ml goat anti-mouse IgG-HRP, γ-chain specific (Cat. No. 401207) Exposure: 1 min Obtained results: Bands at ~30 kDa in lanes 1 and 3
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~28-30 kDa subunit of µ-calpain (calpain-1) and m-calpain (calpain-2).
      BackgroundCalpains are calcium-dependent thiol-proteases that proteolyze a wide variety of cytoskeletal, membrane-associated, and regulatory proteins. The two major isoforms, calpain-1 (µ-form) and calpain-2 (m-form), differ in their calcium requirement for activation. They are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme, whereas the 30 kDa unit is the regulatory subunit and is common to both µ- and m-isozymes.
      HostMouse
      Immunogen speciesHuman Placenta
      Immunogenpurified calpain from human placenta
      Clone28F3
      IsotypeIgG₁
      Speciesbovine, human, not mouse, not rat
      Positive controlMDBK cells
      FormLiquid
      FormulationIn 150 mM sodium chloride, 20 mM sodium phosphate, 50% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling