208910 Anti-Calreticulin (405-417) Rabbit pAb

208910
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ht, H, Mk, M, Porcine, RRbPolyclonal Antibody

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208910-50UL
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      Description
      OverviewRecognizes the ~63 kDa calreticulin protein in heat-shocked HeLa cells.
      Catalogue Number208910
      Brand Family Calbiochem®
      Application Data
      Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesJanicki, S., et al. 1997. J. Cell Biol. 139, 485.
      Wada, I., et al. 1997. EMBO J. 16, 5420.
      Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
      Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
      Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunohistochemistry
      Application NotesImmunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
      Immunocytochemistry (see comments)
      Immunoprecipitation (see comments)
      Immunohistochemistry (see comments)
      Application CommentsDoes not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Canine
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Porcine
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Calreticulin (405-417) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calreticulin (405-417) Rabbit pAb Certificates of Analysis

      TitleLot Number
      208910

      References

      Reference overview
      Janicki, S., et al. 1997. J. Cell Biol. 139, 485.
      Wada, I., et al. 1997. EMBO J. 16, 5420.
      Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
      Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
      Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-April-2009 JSW
      ApplicationImmunoblotting (1:500, mouse/rat; 1:1000, human, chemiluminescent)
      Immunocytochemistry (see comments)
      Immunoprecipitation (see comments)
      Immunohistochemistry (see comments)
      Application Data
      Detection of human calreticulin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysates from HeLa cells (lane 2), L929 cells (lane 3), and Rat2 cells (lane 4). Primary antibody: Anti-Calreticulin (405-417) Rabbit pAb (Cat. No. 208910) (2 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~63 kDa calreticulin protein.
      BackgroundCalreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. Calreticulin, which contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [(C)EEEDVPGQAKDEL] corresponding to amino acids 405-417 of human calreticulin, conjugated to KLH
      IsotypeIgG
      Speciesbovine, canine, hamster, human, monkey, mouse, porcine, rat
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize calreticulin in Drosophila cells. This antibody has been reported to work for immunocytochemistry, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesJanicki, S., et al. 1997. J. Cell Biol. 139, 485.
      Wada, I., et al. 1997. EMBO J. 16, 5420.
      Nauseef, W.F., et al. 1995. J. Biol. Chem. 270, 4741.
      Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
      Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.