208912 Anti-Calreticulin Mouse mAb (FMC75)

208912
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MkMMonoclonal Antibody

Pricing & Availability

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208912-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
      Catalogue Number208912
      Brand Family Calbiochem®
      Application Data
      Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence.
      References
      ReferencesNauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
      Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
      Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
      Positive controlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Flow Cytometry Broquet, A.H., et al. 2003. J. Biol. Chem. 278, 21601. Immunocytochemistry Chen, A., et al. 2002. Biochim. Biophys. Acta 1589, 124. Morello, J.P., et al. 2000. J. Clin. Invest. 105, 887. Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150. Immunohistochemistry Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150.
      Key Applications Immunocytochemistry
      Immunohistochemistry
      Enzyme Immunoassay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Application NotesEnzyme Immunoassay (0.1 µg/ml)
      Immunoblotting (0.5 µg/ml, colorimetric; see application references)
      Immunocytochemistry (see application references)
      Immunohistochemistry (see application references)
      Flow Cytometry (see application references)
      Application CommentsDoes not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena recombinant protein consisting of human calreticulin fused to maltose binding protein
      ImmunogenHuman
      CloneFMC75
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Calreticulin Mouse mAb (FMC75) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calreticulin Mouse mAb (FMC75) Certificates of Analysis

      TitleLot Number
      208912

      References

      Reference overview
      Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
      Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
      Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 JSW
      ApplicationEnzyme Immunoassay (0.1 µg/ml)
      Immunoblotting (0.5 µg/ml, colorimetric; see application references)
      Immunocytochemistry (see application references)
      Immunohistochemistry (see application references)
      Flow Cytometry (see application references)
      Application Data
      Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
      BackgroundCalreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. It contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
      HostMouse
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of human calreticulin fused to maltose binding protein
      CloneFMC75
      IsotypeIgG₁
      Speciesnot E. coli, not bovine, not canine, not guinea pig, not hamster, human, monkey, not mouse, not rat, not salmon
      Positive controlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
      FormLiquid
      FormulationIn PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsDoes not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesNauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
      Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
      Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
      Application referencesImmunoblotting, Flow Cytometry Broquet, A.H., et al. 2003. J. Biol. Chem. 278, 21601. Immunocytochemistry Chen, A., et al. 2002. Biochim. Biophys. Acta 1589, 124. Morello, J.P., et al. 2000. J. Clin. Invest. 105, 887. Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150. Immunohistochemistry Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150.