204903 Anti-Complement 5b-9 Rabbit pAb

204903
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Pricing & Availability

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204903-1MG
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      Description
      OverviewRecognizes complement component complex Cb5-C9.
      Catalogue Number204903
      Brand Family Calbiochem®
      SynonymsAnti-Terminal Complement Complex, Anti-Membrane Attack Complex, Anti-MAC
      References
      ReferencesSchafer, H., et al. 1986. J. Immunol. 137, 1945.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesFrozen Sections Niculescu, F., et al. 2005. Exp. Mol. Pathol. 79, 136.
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      Frozen Sections
      Immunofluorescence
      Paraffin Sections
      Application NotesFrozen Sections (see application references)
      Immunofluorescence (see comments)
      Paraffin Sections (see comments, heat pre-treatment required)
      Radial Immunodiffusion (use undiluted)
      Application CommentsBy immunodiffusion the antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma. Also reported to work for immunofluorescence. This antibody has been shown to work for formalin-fixed paraffin sections; use of citrate buffer and a pressure cooker for 1 min is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified human SC5b-9 complex
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing intial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Complement 5b-9 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Complement 5b-9 Rabbit pAb Certificates of Analysis

      TitleLot Number
      204903

      References

      Reference overview
      Schafer, H., et al. 1986. J. Immunol. 137, 1945.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-December-2007 JSW
      SynonymsAnti-Terminal Complement Complex, Anti-Membrane Attack Complex, Anti-MAC
      ApplicationFrozen Sections (see application references)
      Immunofluorescence (see comments)
      Paraffin Sections (see comments, heat pre-treatment required)
      Radial Immunodiffusion (use undiluted)
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the C5b-C9 complement component complex.
      BackgroundC5b-9 is the cytolytically active glycoprotein complex, comprised of C5b, C6, C7, C8 and C9 complement proteins, formed as a result of complement activation via either the classical or alternative pathways.
      HostRabbit
      Immunogen speciesHuman
      Immunogenpurified human SC5b-9 complex
      IsotypeIgG
      Specieshuman, mouse
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsBy immunodiffusion the antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma. Also reported to work for immunofluorescence. This antibody has been shown to work for formalin-fixed paraffin sections; use of citrate buffer and a pressure cooker for 1 min is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing intial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSchafer, H., et al. 1986. J. Immunol. 137, 1945.
      Application referencesFrozen Sections Niculescu, F., et al. 2005. Exp. Mol. Pathol. 79, 136.