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MABF2819-25UG Anti-Complement C4B Antibody, clone 16D2

Anti-Complement C4B , clone 16D2, Cat. No. MABF2819, is a rat monoclonal antibody that detects Complement C4B and is tested for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABF2819-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2819-25UG
DescriptionAnti-Complement C4B Antibody, clone 16D2
Alternate Names
  • Complement C4-B
  • C4B
  • C4
Background InformationComplement C4-B (UniProt: P01029; also known as C4B) is encoded by the C4b (also known as C4) gene (Gene ID: 12268) in murine species. The complement system provides innate defense against microbial infection and is a complement to antibody mediated immunity. It consists of about thirty-five interacting plasma and membrane associated proteins that contribute to host-defense. It contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as several regulatory membrane-associated proteins that protect host cells from accidental complement attack. Complement C4 is a key component of the complement system and is one of chief constituents of innate immunity for immediate recognition and elimination of invading microbes. It plays an essential role for the functions of both classical and lectin complement pathways. It is a disulfide-bonded three-chain glycoprotein, consisting of an -chain (95 kDa), a β-chain (75 kDa), and a -chain (30 kDa). During complement activation, the -chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the β- and -chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. Dysregulation of classic and lectin complement pathways where complement C4 plays a vital role can lead to complement-mediated autoimmune diseases. (Ref.: Wang, H., and Liu, M. (2021). Front. Immunol. 12; Article 694928; Kremmer, E., et al. (1990). Hybridoma. 9(4); 309-317).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Complement C4B , clone 16D2, Cat. No. MABF2819, is a rat monoclonal antibody that detects Complement C4B and is tested for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Western Blotting
  • Immunofluorescence
  • ELISA
Application NotesTested Applications

ELISA Analysis: A representative lot detected Complement C4B in ELISA applications (Yilmaz, M., et. al. (2021). Nat Neurosci. 24(2); 214-224).

Immunohistochemistry Applications: A representative lot detected Complement C4B in Immunohistochemistry applications (Kremmer, E., et al. (1990). Hybridoma. 9(4): 309-17).

Immunofluorescence Analysis: A representative lot detected Complement C4B in Immunofluorescence applications (Simoni, L., et. al. (2020). Cell Rep. 33(5): 108330).

Western Blotting Analysis: A representative lot detected Complement C4B in Western Blotting applications (Kremmer, E., et al. (1990). Hybridoma. 9(4):309-17).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenC57BL/6 thymocytes incubated with saturating amounts of a rat anti-Thy-1 monoclonal antibody and C57BL/6 mouse serum
EpitopeUnknown
Clone16D2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 16D2 is a rat monoclonal antibody that detects mouse Complement C4B.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • C4b
Purification MethodProtein G purified
UniProt Number
Molecular Weight192.92 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in Mouse spleen tissue sections.

Immunohistochemistry Applications: A representative lot detected Mouse C4B in frozen Mouse spleen tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2819-25UG 04065270647139