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DR1014 Anti-HAUSP Rabbit pAb

Anti-HAUSP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Herpes-Associated Ubiquitin-Specific Protease, Anti-USP7

DR1014
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~135 kDa HAUSP protein in HeLa cell nuclear extracts.
Catalogue NumberDR1014
Brand Family Calbiochem®
SynonymsAnti-Herpes-Associated Ubiquitin-Specific Protease, Anti-USP7
Application Data
A. Detection of human HAUSP by immunoblotting. Sample: Whole cell extracts from HeLa cells (50 µg). Primary antibody: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (1:3000) Detection: chemiluminescence B. Detection of human HAUSP by immunoprecipitation followed by immunoblotting. Sample: Nuclear extract from HeLa cells (1 mg). Antibody for IP: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (2 µg/mg lysate). Immunoblotting conditions: Same as panel A with 10 µg HeLa nuclear extract used as the control.
References
ReferencesLi, M., et al. 2004. Mol. Cell 13, 879.
Muyang, L., et al. 2002. Nature 416, 648.
Vugmeyster, Y., et al. 2002. Mol. Immunol. 39, 431.
Product Information
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Positive controlHeLa cell nuclear extracts
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000-1:10,000)
Immunoprecipitation (1-4 µg/mg lysate)
Application CommentsHAUSP protein is reported to stabilize p53 through the deubiquitination of p53. HAUSP has also been reported to be cleaved by caspase-3. May recognize additional bands, possibly corresponding to HAUSP cleavage products. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids encoded within exon 3 of human HAUSP
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1014 0

Documentation

Anti-HAUSP Rabbit pAb Certificates of Analysis

TitleLot Number
DR1014

References

Reference overview
Li, M., et al. 2004. Mol. Cell 13, 879.
Muyang, L., et al. 2002. Nature 416, 648.
Vugmeyster, Y., et al. 2002. Mol. Immunol. 39, 431.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
SynonymsAnti-Herpes-Associated Ubiquitin-Specific Protease, Anti-USP7
ApplicationImmunoblotting (1:1000-1:10,000)
Immunoprecipitation (1-4 µg/mg lysate)
Application Data
A. Detection of human HAUSP by immunoblotting. Sample: Whole cell extracts from HeLa cells (50 µg). Primary antibody: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (1:3000) Detection: chemiluminescence B. Detection of human HAUSP by immunoprecipitation followed by immunoblotting. Sample: Nuclear extract from HeLa cells (1 mg). Antibody for IP: Anti-HAUSP Rabbit pAb (Cat. No. DR1014) (2 µg/mg lysate). Immunoblotting conditions: Same as panel A with 10 µg HeLa nuclear extract used as the control.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~135 kDa HAUSP protein.
Backgroundp53, a tumor suppressor phosphoprotein is present at low concentrations in normal cells. The low concentration is maintained by MDM2-mediated ubiquitination and resulting proteolysis. HAUSP, an ubiquitin hydrolase, is thought to regulate MDM2 and help stabilize p53. When cells are subjected to stress, such as hypoxia or UV radiation, p53 is activated, its protein levels are elevated and its ubiquitin-dependent degradation is blocked. The resulting increase in p53 leads either to the induction of cell cycle arrest or apoptosis.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids encoded within exon 3 of human HAUSP
IsotypeIgG
Specieshuman
Positive controlHeLa cell nuclear extracts
FormLiquid
FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsHAUSP protein is reported to stabilize p53 through the deubiquitination of p53. HAUSP has also been reported to be cleaved by caspase-3. May recognize additional bands, possibly corresponding to HAUSP cleavage products. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Highly Toxic
ReferencesLi, M., et al. 2004. Mol. Cell 13, 879.
Muyang, L., et al. 2002. Nature 416, 648.
Vugmeyster, Y., et al. 2002. Mol. Immunol. 39, 431.