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ABC1451-100UG Anti-PDGF-D

Anti-PDGF-D, Cat. No. ABC1451, is a rabbit polyclonal antibody that detects Platelet-derived growth factor D in human cells and has been tested for use in Western Blotting.

Anti-PDGF-D MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: PDGFD, IEGF, SCDGFB, MSTP036 UniProt Number: Q9GZP0
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ABC1451-100UG
100 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABC1451-100UG
DescriptionAnti-PDGF-D
Alternate Names
  • Platelet-derived growth factor D
  • Iris-expressed growth factor
  • Spinal cord-derived growth factor B
  • SCDGF-B
Background InformationPlatelet-derived growth factor D (UniProt: Q9GZP0; also known as PDGF-D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B) is encoded by the PDGFD (also known as IEGF, SCDGFB, MSTP036, UNQ1899/PRO4345) gene (Gene ID: 80310) in human. Four PDGF ligands known as PDGF-A, PDGF-B, PDGF-C, and PDGF-D have been described that exert their effect by binding to their cognate receptors. PDGF-D is expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen, and colon. PDGF-D is synthesized with a signal peptide (aa 1-18), which is subsequently removed. The latent form of PDGF-D contains a CUB domain at its N-terminal region, a growth factor domain and a hinge region in between. The latent form is activated by the proteolytic action of matriptase that removes the CUB domain, which results in unmasking of the receptor-binding epitopes on the core domain. YR247GR249S within the PDGF-D hinge region is shown to be the cleavage site for removal of the inhibitory CUB domain. PDGF-D plays an important role in wound healing and induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. PDGF-D also induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. (Ref.: Huang, W., and Kim, H-R, C (2015). J. Biol. Chem. 290(14); 9162-9170).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS (pH 7.4) with 0.09% sodium azide.
Applications
ApplicationAnti-PDGF-D, Cat. No. ABC1451, is a rabbit polyclonal antibody that detects Platelet-derived growth factor D in human cells and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected PDGF-D in human recombinant-PDGF D (Huang, W., et. al. (2015). J Biol Chem. 290(14):9162-70) and LNCaP cells expressing PDGF-D, BS-C-1 cells transfected with the plasmid pTF7-PDGF D:HIS, recombinant PDGF-D (Ustach, C.V., et. al. (2004). Cancer Res. 64(5):1722-9).

Western Blotting Analysis: A representative lot detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Biological Information
ImmunogenA linear peptide corresponding to 18 amino acids from the C-terminal half of human Platelet-derived growth factor D.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Platelet-derived growth factor D in human cells. It targets an epitope within the growth factor domain from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rabbit based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDGFD
  • IEGF
  • SCDGFB
  • MSTP036
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~90 kDa Full Length PDGF D dimer, ~52 kDa PDGF D hemi-dimer,~32 kDa growth factor dimer in the non-reducing conditions; 42.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting condition media collected from LNCaP cells transfected with PDGF-D expression vector.

Western Blotting Analysis: 2 µg/mL of this antibody detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1451-100UG 04054839460081