203118 Biotin-NHS, Water-Soluble

203118
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203118-50MG
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      Plastic ampoule 50 mg
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      Description
      OverviewReacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.
      Catalogue Number203118
      Brand Family Calbiochem®
      SynonymsSulfobiotin-NHS, Sulfosuccinimidobiotin
      References
      ReferencesLisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.
      Product Information
      FormOff-white solid
      Hill FormulaC₁₄H₁₈N₃O₈S₂ · Na
      Chemical formulaC₁₄H₁₈N₃O₈S₂ · Na
      Quality LevelMQ100
      Applications
      Biological Information
      Purityhomogeneous single spot by TLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Biotin-NHS, Water-Soluble SDS

      Title

      Safety Data Sheet (SDS) 

      Biotin-NHS, Water-Soluble Certificates of Analysis

      TitleLot Number
      203118

      References

      Reference overview
      Lisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-May-2008 RFH
      SynonymsSulfobiotin-NHS, Sulfosuccinimidobiotin
      DescriptionWater-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.
      FormOff-white solid
      Recommended reaction conditions
      Buffers containing amines (such as Tris or glycine) should be avoided because they can compete with the reaction. It is also very important that the stock solution be prepared just prior to addition of the reaction mixture, because these compounds are prone to hydrolysis by water. Water-soluble crosslinkers can be dissolved in water or buffer at a concentration of 10-25 mM. Phosphate buffers may precipitate sulfo-N-hydroxysuccinimide (sulfo-NHS) containing compounds. In addition, the solid crosslinker can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions, but can be minimized by using more concentrated protein solution (greater than 5 mg/ml). If you prefer to work with stock solutions, we recommend that you dissolve the crosslinker in anhydrous DMSO at a concentration of 10-25 mM. This solution can then be diluted into the buffer of your choice just prior to use. General Handling 1. Store desiccated at 4°C. 2. Warm contents to room temperature completely before opening. Allow at least 30 min. 3. Wipe the vial to remove any moisture. 4. After opening, store under nitrogen. 5. Protect from moisture.
      Chemical formulaC₁₄H₁₈N₃O₈S₂ · Na
      Purityhomogeneous single spot by TLC
      SolubilitydH₂O (4 mg/ml) or DMSO (10 mg/ml)
      Storage +2°C to +8°C
      Protect from moisture
      Do Not Freeze Ok to freeze
      Special InstructionsUnstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesLisanti, M.P., et al. 1989. J. Cell Biol. 109, 2145.